Improving the performance of hypercrosslinked monolithic stationary phases and their application in separations of polar compounds
Provider: Grantová agentura ČR
Programme: Postdoktorské projekty (řešení od 1.1.2012)
Implementation period: 01.01.12 - 31.12.14
Workplace:
Description:
The aim of this project is to prepare and optimize hypercrosslinked poly(styrene-vinylbenzylchloride-divinylbenzene) monolithic stationary phases. The internal structure of hypercrosslinked monoliths with relatively thin hypercrosslinked layer on the pore surface evokes the structure of super cially porous particles. The optimization of this porous layer can bring the properties of hypercrosslinked monoliths near the properties of currently available highly ecient columns packed with super cially porous particles. The tailored chemical modi cation of the reactive groups located at the pore surface is additionally proposed. This modi cation should enable formation of libraries of new types of stationary phases that can be prepared from just a single optimized generic monolithic structure. The project proposes the preparation of stationary phases with functionalities suitable for hydrophilic interaction liquid chromatography. The results gained will provide the separation of complex samples containing polar compounds, such as drugs, metabolites, and other natural compounds.
The aim of this project is to prepare and optimize hypercrosslinked poly(styrene-vinylbenzylchloride-divinylbenzene) monolithic stationary phases. The internal structure of hypercrosslinked monoliths with relatively thin hypercrosslinked layer on the pore surface evokes the structure of super cially porous particles. The optimization of this porous layer can bring the properties of hypercrosslinked monoliths near the properties of currently available highly ecient columns packed with super cially porous particles. The tailored chemical modi cation of the reactive groups located at the pore surface is additionally proposed. This modi cation should enable formation of libraries of new types of stationary phases that can be prepared from just a single optimized generic monolithic structure. The project proposes the preparation of stationary phases with functionalities suitable for hydrophilic interaction liquid chromatography. The results gained will provide the separation of complex samples containing polar compounds, such as drugs, metabolites, and other natural compounds.
