Two-dimensional separations in liquid media
Provider: Grantová agentura ČR
Programme: Standardní projekty (řešení od 1.1.2012)
Implementation period: 01.01.12 - 31.12.14
Workplace:
Description:
New two-dimensional separations will be developed with high peak capacity and improved compatibility of on-line coupled reverse-phase (RP) and hydrophilic interaction (HILIC) LC systems with broad separation range, using second-dimension gradients matching the elution strength in rst dimension. New ecient monolithic columns with dual HILIC-RP retention mechanism will be synthesized for 2D HPLC applications. On-line enrichment modulation of fraction transfer between the two LC dimensions will improve the detection limits. In coupled HPLC-MEKC 2D separation systems, in-capillary sample enrichment will be improved by compensation for the e ects of organic solvent in the fractions transferred from the HPLC column using modi cations of the background electrolyte. Software will be developed for quantitative evaluation of two-dimensional data matrix with improved analyte tracking possibilities by implementation of multi-channel UV, MS and electrochemical detection. The two-dimensional separation methods developed will be applied in the analysis of plant extracts and food samples.
New two-dimensional separations will be developed with high peak capacity and improved compatibility of on-line coupled reverse-phase (RP) and hydrophilic interaction (HILIC) LC systems with broad separation range, using second-dimension gradients matching the elution strength in rst dimension. New ecient monolithic columns with dual HILIC-RP retention mechanism will be synthesized for 2D HPLC applications. On-line enrichment modulation of fraction transfer between the two LC dimensions will improve the detection limits. In coupled HPLC-MEKC 2D separation systems, in-capillary sample enrichment will be improved by compensation for the e ects of organic solvent in the fractions transferred from the HPLC column using modi cations of the background electrolyte. Software will be developed for quantitative evaluation of two-dimensional data matrix with improved analyte tracking possibilities by implementation of multi-channel UV, MS and electrochemical detection. The two-dimensional separation methods developed will be applied in the analysis of plant extracts and food samples.
