Study of a new mechanism of acetaminophen toxicity in liver and possibilities of therapy
Provider: Ministerstvo zdravotnictví
Programme: Rezortní program výzkumu a vývoje - MZ III (2010 - 2015)
Implementation period: 01.05.13 - 31.12.15
Workplace:
Fakulta chemicko-technologická - Katedra biologických a biochemických věd
Investigator: Roušar TomášTeam member: Česla Petr
Description:
The aim of the project is the description of a newly found toxic mechanism of acetaminophen toxicity that contributes to hepatotoxic effect of acetaminophen (APAP); our aim has been stated regarding our published and also unpublished data. After APAP overdose, the specific metabolite is produced in the cell. This compound has been considered as non-toxic. In this project, we intend to synthesize and purify it in sufficient quantity. Consequently, we will use it to detailed study of its effects in cultured hepatocytes in vitro and in vivo in mice and rats.
The aim of the project is the description of a newly found toxic mechanism of acetaminophen toxicity that contributes to hepatotoxic effect of acetaminophen (APAP); our aim has been stated regarding our published and also unpublished data. After APAP overdose, the specific metabolite is produced in the cell. This compound has been considered as non-toxic. In this project, we intend to synthesize and purify it in sufficient quantity. Consequently, we will use it to detailed study of its effects in cultured hepatocytes in vitro and in vivo in mice and rats.
