Modern instrumental methods in analysis of materials, food and biological samples
Provider: Univerzita Pardubice
Programme: Studentská grantová soutěž
Implementation period: 01.01.13 - 31.12.13
Workplace:
Fakulta chemicko-technologická - Katedra analytické chemie
Investigator: Ventura Karel
Description:
In the field of the separation methods, the new separation systems with the high efficiency and selectivity will be developed for HPLC in uni-dimensional and two-dimensional separation setup. Experiments will be focused on the optimization of fast gradients using UHPLC technique, development of the two-dimensional separations using capillary electrophoresis, application of developed separation methods for the analysis of surfactants and complex characterization of the naturally occurring compounds. The development of new methods for lipidomic characterization of biological samples with UHPLC/MS and MALDI-MS including the mass spectrometric imaging of cancer tissues of oncologic patients will continue in the field of mass spectrometry. The research of modern extraction and microextraction techniques will continue for the isolation of food constituents, plant samples, biological samples and explosives followed by the analysis of extracts using separation techniques with the various types of detectors (GC/FID, GC/MS, HPLC/UV/MS). The methods for direct analysis of suspensions using atomic absorption spectrometry as the time-saving alternative to the standard procedures based on wet mineralization will be developed. The other research areas will include the development and application of non-mercury electrodes, especially those based on the carbon materials; development and application of the amperometric biosensors; electrocatalytic determination of the compounds using carbon paste electrode modified by multiwall carbon nanotubes and the application of carbon electrodes on determination of the quality of edible oils.
In the field of the separation methods, the new separation systems with the high efficiency and selectivity will be developed for HPLC in uni-dimensional and two-dimensional separation setup. Experiments will be focused on the optimization of fast gradients using UHPLC technique, development of the two-dimensional separations using capillary electrophoresis, application of developed separation methods for the analysis of surfactants and complex characterization of the naturally occurring compounds. The development of new methods for lipidomic characterization of biological samples with UHPLC/MS and MALDI-MS including the mass spectrometric imaging of cancer tissues of oncologic patients will continue in the field of mass spectrometry. The research of modern extraction and microextraction techniques will continue for the isolation of food constituents, plant samples, biological samples and explosives followed by the analysis of extracts using separation techniques with the various types of detectors (GC/FID, GC/MS, HPLC/UV/MS). The methods for direct analysis of suspensions using atomic absorption spectrometry as the time-saving alternative to the standard procedures based on wet mineralization will be developed. The other research areas will include the development and application of non-mercury electrodes, especially those based on the carbon materials; development and application of the amperometric biosensors; electrocatalytic determination of the compounds using carbon paste electrode modified by multiwall carbon nanotubes and the application of carbon electrodes on determination of the quality of edible oils.
