Development of microfluidic interfaces for multidimensional liquid phase separation/mass spectrometry analysis of complex biological samples
Provider: Grantová agentura ČR
Programme: Standardní projekty (řešení od 1.1.2014)
Implementation period: 01.01.14 - 31.12.16
Workplace:
Fakulta chemicko-technologická - Katedra analytické chemie
Investigator: Česla PetrTeam member: Fischer Jan
Description:
The proposed project deals with development of new instrumentation for glycoproteomic analysis. New microfluidic interfaces for on-line coupling of liquid phase separations such as capillary liquid chromatography and capillary electrophoresis will be developed. The procedure for systematic optimization of the separation conditions will be developed. The monolithic materials will be modified with nanoparticles, proteolytic and glycolytic enzymes as well as affinity ligands and integrated into the multidimensional separation systems. The coupling with mass spectrometry will be employed for identification and characterization of individual analytes. While the primary target in the proposed project will be the analysis of protein glycosylation associated with cancer and food allergy, the developed technology is expected to be of general use not only for glycoproteomic analysis, but also applicable for general characterization of complex samples.
The proposed project deals with development of new instrumentation for glycoproteomic analysis. New microfluidic interfaces for on-line coupling of liquid phase separations such as capillary liquid chromatography and capillary electrophoresis will be developed. The procedure for systematic optimization of the separation conditions will be developed. The monolithic materials will be modified with nanoparticles, proteolytic and glycolytic enzymes as well as affinity ligands and integrated into the multidimensional separation systems. The coupling with mass spectrometry will be employed for identification and characterization of individual analytes. While the primary target in the proposed project will be the analysis of protein glycosylation associated with cancer and food allergy, the developed technology is expected to be of general use not only for glycoproteomic analysis, but also applicable for general characterization of complex samples.
