Development of an multifunctional monolithic capillary column with integrated sample focusing, separation, and on-column electrochemical detection
Provider: Grantová agentura ČR
Programme: Standardní projekty (řešení od 1.1.2014)
Implementation period: 01.01.14 - 31.12.16
Workplace:
Team member: Metelka Radovan
Description:
In this project we propose preparation of a multifunctional capillary device based on organic polymer monoliths. We propose spatially constrained surface modification of prepared monolithic columns to integrate sample focusing, separation, and on-column electrochemical detection. The surface grafting will be used to prepare segments with different polarity and chemistry. Monolithic surface will be also modified with polymers responding to external stimuli (e.g. temperature) to prepare so called smart stationary phases. An electrochemical detector will be directly incorporated into the porous structure of monolithic column, either by embedding the microelectrodes at the end of capillary column, or by preparation of desired electrodes via formation of porous metal surfaces with the use of electroless deposition techniques. We propose application of developed device in direct analysis of neurotransmitters as well as comprehensive online coupling with microdialysis sampling in pharmacology, drug delivery, drug kinetics, and tissue metabolism studies.
In this project we propose preparation of a multifunctional capillary device based on organic polymer monoliths. We propose spatially constrained surface modification of prepared monolithic columns to integrate sample focusing, separation, and on-column electrochemical detection. The surface grafting will be used to prepare segments with different polarity and chemistry. Monolithic surface will be also modified with polymers responding to external stimuli (e.g. temperature) to prepare so called smart stationary phases. An electrochemical detector will be directly incorporated into the porous structure of monolithic column, either by embedding the microelectrodes at the end of capillary column, or by preparation of desired electrodes via formation of porous metal surfaces with the use of electroless deposition techniques. We propose application of developed device in direct analysis of neurotransmitters as well as comprehensive online coupling with microdialysis sampling in pharmacology, drug delivery, drug kinetics, and tissue metabolism studies.
