Modern instrumental methods in analysis of materials, food and biological samples
Provider: Univerzita Pardubice
Programme: Studentská grantová soutěž
Implementation period: 01.01.14 - 31.12.14
Workplace:
Fakulta chemicko-technologická - Katedra analytické chemie
Investigator: Ventura KarelTeam member: Fischer Jan | Holčapek Michal | Švancara Ivan | Červenka Libor | Česlová Lenka | Meloun Milan | Adam Martin
Description:
In the field of the separation methods, the new separation systems with the high efficiency and selectivity will be developed for HPLC in uni-dimensional and two-dimensional separation setup. Experiments will be focused on the optimization of fast gradients using UHPLC technique, development of the two-dimensional separations using capillary electrophoresis, application of developed separation methods for the analysis of surfactants and complex characterization of the naturally occurring compounds. The development of new methods for lipidomic characterization of biological samples with UHPLC-MS, GC-MS and MALDI-MS including the mass spectrometric imaging of cancer tissues obtained during oncologic surgeries, neighbouring healthy tissues, plasma and urine. The research of extraction and microextraction techniques will continue for the isolation of food constituents and plant samples followed by the analysis of extracts using separation techniques with the various types of detectors (GC-FID/MS and HPLC/UV/MS). Research will be focused on the utilization of matrix chemical modifiers for elimination of selected interfering polyatomic ions at real multi-compound analysis. By means of high resolution instrumentation the identification and explanation of interfering mechanisms for selected interferes and their elimination will be tested. The other research areas will include the development and application of non-mercury electrodes, especially those based on the carbon materials and their application for analysis of environmental pollutants and biologically active compounds, methods for rutin and acrylamide determination in real food samples or food supplements.
In the field of the separation methods, the new separation systems with the high efficiency and selectivity will be developed for HPLC in uni-dimensional and two-dimensional separation setup. Experiments will be focused on the optimization of fast gradients using UHPLC technique, development of the two-dimensional separations using capillary electrophoresis, application of developed separation methods for the analysis of surfactants and complex characterization of the naturally occurring compounds. The development of new methods for lipidomic characterization of biological samples with UHPLC-MS, GC-MS and MALDI-MS including the mass spectrometric imaging of cancer tissues obtained during oncologic surgeries, neighbouring healthy tissues, plasma and urine. The research of extraction and microextraction techniques will continue for the isolation of food constituents and plant samples followed by the analysis of extracts using separation techniques with the various types of detectors (GC-FID/MS and HPLC/UV/MS). Research will be focused on the utilization of matrix chemical modifiers for elimination of selected interfering polyatomic ions at real multi-compound analysis. By means of high resolution instrumentation the identification and explanation of interfering mechanisms for selected interferes and their elimination will be tested. The other research areas will include the development and application of non-mercury electrodes, especially those based on the carbon materials and their application for analysis of environmental pollutants and biologically active compounds, methods for rutin and acrylamide determination in real food samples or food supplements.
