The development of the methodology for determination of the cytostatic efect of new metalotherapeutics
Provider: Ministerstvo školství, mládeže a tělovýchovy
Programme: Interní rozvojová soutěž
Implementation period: 01.01.15 - 31.12.15
Workplace:
Team member: Vinklárek Jaromír
Description:
The goal of the project is to summarize and develop the methodology for determination of inhibition and the study of the mechanism of cytostatic effect of the new metallotherapeutics. The testing of certain compounds with a Pt, V, Mo as a central atom will be done on MOLT-4, HL-60 and Jurkat leukemia cell lines. Analytic and spectral methods such as the flow cytometry or the Western blotting combined with the SDS-PAGE and the immunochemical detection will be used to describe the mechanism of the cytotoxic impact. The obtained knowledge and the processed materials will serve to implement new laboratory exercises on master's degree studies. Some bachelor's and master's thesis topics will be further created, concerning the preparation, the description and the determination of the biologic activity of the transition metal complexes.
The goal of the project is to summarize and develop the methodology for determination of inhibition and the study of the mechanism of cytostatic effect of the new metallotherapeutics. The testing of certain compounds with a Pt, V, Mo as a central atom will be done on MOLT-4, HL-60 and Jurkat leukemia cell lines. Analytic and spectral methods such as the flow cytometry or the Western blotting combined with the SDS-PAGE and the immunochemical detection will be used to describe the mechanism of the cytotoxic impact. The obtained knowledge and the processed materials will serve to implement new laboratory exercises on master's degree studies. Some bachelor's and master's thesis topics will be further created, concerning the preparation, the description and the determination of the biologic activity of the transition metal complexes.
