Modern instrumental methods in analysis of materials, food and biological samples
Provider: Univerzita Pardubice
Programme: Studentská grantová soutěž
Implementation period: 01.01.15 - 31.12.15
Workplace:
Fakulta chemicko-technologická - Katedra analytické chemie
Investigator: Ventura KarelTeam member: Fischer Jan | Holčapek Michal | Červenka Libor | Meloun Milan | Švancara Ivan | Adam Martin | Česlová Lenka | Bajerová Petra
Description:
In the field of the separation methods, the new separation systems with the high efficiency and selectivity will be developed for HPLC in uni-dimensional and two-dimensional separation setup. There will be prepared and tested now types of monolithic capillary columns suitable for separations in HILIC systems. Experiments will be focused on the optimization of fast gradients using UHPLC technique and development of the two-dimensional separations using capillary and microchip electrophoresis. The interface for combination of liquid chromatography and electromigration techniques by means of 3D printing and microfluidics will be developed. These methods will be applied to the analysis of natural and synthetic surfactants and complex characterization of the naturally occurring compounds in plant matrices. For selected cancer types there will be processed and analysed samples of cancer tissues together with neighbouring normal tissues by methods created for quantitative lipidomic analysis by SFC/MS, shotgun MS or MALDI-MS. New methods for analysis of glycosphingolipids by ESI-MS will be developed. The research of modern extraction approaches (USE, SPME, SBSE, etc.) will continue for the isolation of food constituents and plant samples followed by the analysis by separation techniques with the various types of detectors (GC-FID/ECD/MS and HPLC/UV/MS/ECD). Emphasis will be placed on the ?Green Analytical Chemistry? principles. The other research areas will include the development and the testing of electrodes based on new carbon types and similar materials for electroanalytical determination of biologically active compounds belonging to the natural psychotropic compounds. Development of electrometric enzyme biosensors based on carbon nanotubes will continue. There will be studied various configurations of electrodes modified by bismuth and bismuthyl chloride with respect to electroanalytical behavior for determination of trace content of heavy metal ions in water and sediment pores. Attention will be given to applications of potentiometric sensors for the surfactants determination. Research will be focused on the study of acrylamide reactions with selected organic compounds with attention to appropriate electrochemical properties. The effect of relative air humidity on the beverage prepared from Ilex paraguariensis quality will be studied. The aim of research will be also the verification of claims concerning minimally treated foods, especially their antioxidant properties, effect on the microorganism contents and selected properties of fats. Research will be focused on the development and the optimisation of environmentally friendly, less time consuming and cheaper sample pretreatment methods for the needs of following analysis by atomic spectrometry methods. Also there will be tested new approaches for corrections of spectral influences in AAS and ICP-MS analysis.
In the field of the separation methods, the new separation systems with the high efficiency and selectivity will be developed for HPLC in uni-dimensional and two-dimensional separation setup. There will be prepared and tested now types of monolithic capillary columns suitable for separations in HILIC systems. Experiments will be focused on the optimization of fast gradients using UHPLC technique and development of the two-dimensional separations using capillary and microchip electrophoresis. The interface for combination of liquid chromatography and electromigration techniques by means of 3D printing and microfluidics will be developed. These methods will be applied to the analysis of natural and synthetic surfactants and complex characterization of the naturally occurring compounds in plant matrices. For selected cancer types there will be processed and analysed samples of cancer tissues together with neighbouring normal tissues by methods created for quantitative lipidomic analysis by SFC/MS, shotgun MS or MALDI-MS. New methods for analysis of glycosphingolipids by ESI-MS will be developed. The research of modern extraction approaches (USE, SPME, SBSE, etc.) will continue for the isolation of food constituents and plant samples followed by the analysis by separation techniques with the various types of detectors (GC-FID/ECD/MS and HPLC/UV/MS/ECD). Emphasis will be placed on the ?Green Analytical Chemistry? principles. The other research areas will include the development and the testing of electrodes based on new carbon types and similar materials for electroanalytical determination of biologically active compounds belonging to the natural psychotropic compounds. Development of electrometric enzyme biosensors based on carbon nanotubes will continue. There will be studied various configurations of electrodes modified by bismuth and bismuthyl chloride with respect to electroanalytical behavior for determination of trace content of heavy metal ions in water and sediment pores. Attention will be given to applications of potentiometric sensors for the surfactants determination. Research will be focused on the study of acrylamide reactions with selected organic compounds with attention to appropriate electrochemical properties. The effect of relative air humidity on the beverage prepared from Ilex paraguariensis quality will be studied. The aim of research will be also the verification of claims concerning minimally treated foods, especially their antioxidant properties, effect on the microorganism contents and selected properties of fats. Research will be focused on the development and the optimisation of environmentally friendly, less time consuming and cheaper sample pretreatment methods for the needs of following analysis by atomic spectrometry methods. Also there will be tested new approaches for corrections of spectral influences in AAS and ICP-MS analysis.
