Modern instrumental methods in analysis of materials, food and biological samples
Provider: Univerzita Pardubice
Programme: Studentská grantová soutěž
Implementation period: 01.01.16 - 31.12.16
Workplace:
Fakulta chemicko-technologická - FCHT
Investigator: Ventura KarelTeam member: Adam Martin | Červenka Libor | Fischer Jan | Holčapek Michal | Česlová Lenka | Meloun Milan | Švancara Ivan | Česla Petr | Bajerová Petra
Description:
Separation properties of diol and amidic columns for separation of phenolic compounds and flavones in double retention mechanism systems will be investigated. Possible utilisation of combination of various separation mechanisms on polar and nonpolar columns for development of novel two-dimensional separation methods will be studied. Possibilities of gradient retention characteristics predictions based on isocratic model in hydrophilic interaction chromatography for separation of oligosaccharide derivates by micro liquid chromatography will be tested. Development of microfluidic interface for combination of HPLC an capillary electrophoresis in on-line system will be finished. Methods for determination of critical micellar concentration of anionic surfactants and their mixtures with non-ionic surfactants will be developed. Influence of organic solvents presence on accuracy of critical micellar concentration determination will be investigated. Development of LC-MS methods will continue for analysis of glycosphingolipids. Works on comparison of lipidomic quantitative methods for analysis of plasma samples by means of SFC-MS, shotgun MS and HILIC-MS will be finished. Samples of cancer tissues and neighbouring normal tissues from patients with lung cancer will be measured by shotgun MS and differences in lipidomic contents will be statistically evaluated. Research will be focused on the optimisation of extraction and separation techniques for analysis of biologically significant compounds contained if natural matrices. Changes of these components contents depending on the controlled contamination of barley by selected fungal species will be monitored. Modern extraction and microextraction approaches for preconcentration of biologically active compounds in the brewing malts followed by HPLC-DAD/ECD or GC-MS analysis will be studied. SFE conditions will be optimised for GC-MS analysis of volatile compounds in rose samples. Post-explosion combustion products of improvised explosives will be analysed by GC-MS method with chemical ionisation. Stability of quercetin in model system of leaves stored at various temperatures and relative air humidities will be tested. Content of antioxidant enzymes and total antioxidant capacity of minimally treated foods will be studied. In the field of carbon paste electrodes the research will be focused on the electrochemical methods for determination of significant contaminants or biologically active compounds in foodstuffs. Possibilities of acrylamide, proline or amygdaline determination will be investigated. Applications of carbon paste and printed electrodes for determination of antioxidants in foodstuffs and cosmetic products, development and application of miniaturised electrochemical detectors for HPLC analysis of neurotransmitters and utilisation of film electrodes for detection of quantum dots from metal sulphides will be tested. Development of novel configuration of carbon type electrodes and their testing focused on electroanalytical determination of biologically active compounds, especially natural alkaloids, will continue together with development of electrochemical biosensors using carbon nanotubes. In the field of isotachophoresis the behaviour of cyanides and cyan complexes will be investigated. New approaches for correction of interfering effects in atomic spectral analysis will be tested. Attention will be paid to speciation analysis for determination of chemical forms of elements in solid materials. Environmentally friendly, less time consuming and cheaper sample pretreatment methods of inorganic elemental analysis of selected sample types will be developed and optimised by means of modern methods of atomic spectrometry and effective and efficient tools of univariate and multivariate data processing.
Separation properties of diol and amidic columns for separation of phenolic compounds and flavones in double retention mechanism systems will be investigated. Possible utilisation of combination of various separation mechanisms on polar and nonpolar columns for development of novel two-dimensional separation methods will be studied. Possibilities of gradient retention characteristics predictions based on isocratic model in hydrophilic interaction chromatography for separation of oligosaccharide derivates by micro liquid chromatography will be tested. Development of microfluidic interface for combination of HPLC an capillary electrophoresis in on-line system will be finished. Methods for determination of critical micellar concentration of anionic surfactants and their mixtures with non-ionic surfactants will be developed. Influence of organic solvents presence on accuracy of critical micellar concentration determination will be investigated. Development of LC-MS methods will continue for analysis of glycosphingolipids. Works on comparison of lipidomic quantitative methods for analysis of plasma samples by means of SFC-MS, shotgun MS and HILIC-MS will be finished. Samples of cancer tissues and neighbouring normal tissues from patients with lung cancer will be measured by shotgun MS and differences in lipidomic contents will be statistically evaluated. Research will be focused on the optimisation of extraction and separation techniques for analysis of biologically significant compounds contained if natural matrices. Changes of these components contents depending on the controlled contamination of barley by selected fungal species will be monitored. Modern extraction and microextraction approaches for preconcentration of biologically active compounds in the brewing malts followed by HPLC-DAD/ECD or GC-MS analysis will be studied. SFE conditions will be optimised for GC-MS analysis of volatile compounds in rose samples. Post-explosion combustion products of improvised explosives will be analysed by GC-MS method with chemical ionisation. Stability of quercetin in model system of leaves stored at various temperatures and relative air humidities will be tested. Content of antioxidant enzymes and total antioxidant capacity of minimally treated foods will be studied. In the field of carbon paste electrodes the research will be focused on the electrochemical methods for determination of significant contaminants or biologically active compounds in foodstuffs. Possibilities of acrylamide, proline or amygdaline determination will be investigated. Applications of carbon paste and printed electrodes for determination of antioxidants in foodstuffs and cosmetic products, development and application of miniaturised electrochemical detectors for HPLC analysis of neurotransmitters and utilisation of film electrodes for detection of quantum dots from metal sulphides will be tested. Development of novel configuration of carbon type electrodes and their testing focused on electroanalytical determination of biologically active compounds, especially natural alkaloids, will continue together with development of electrochemical biosensors using carbon nanotubes. In the field of isotachophoresis the behaviour of cyanides and cyan complexes will be investigated. New approaches for correction of interfering effects in atomic spectral analysis will be tested. Attention will be paid to speciation analysis for determination of chemical forms of elements in solid materials. Environmentally friendly, less time consuming and cheaper sample pretreatment methods of inorganic elemental analysis of selected sample types will be developed and optimised by means of modern methods of atomic spectrometry and effective and efficient tools of univariate and multivariate data processing.
