Modern instrumental methods in analysis of materials, food and biological samples
Provider: Univerzita Pardubice
Programme: Studentská grantová soutěž
Implementation period: 01.01.17 - 31.12.17
Workplace:
Fakulta chemicko-technologická - FCHT
Investigator: Ventura KarelTeam member: Adam Martin | Červenka Libor | Fischer Jan | Holčapek Michal | Česlová Lenka | Meloun Milan | Švancara Ivan | Česla Petr | Bajerová Petra
Description:
New capillary monolithic column will be prepared and their chromatographic properties studied in order to investigate their possible utilisation for separation of phenolic compounds and flavones, nucleosides and other compounds in systems based on the HILIC and RP retention mechanisms. Research of the physico-chemical properties determination of micellar systems will continue. In this case, various methods for determination of critical micellar concentration of sodium alkylsulphates and volatile perfluorinated acids as surfactants suitable for connection with mass spectrometry will be studied. Application of two-dimensional separation techniques such as capillary chromatography and capillary electrophoresis followed by fluorescence detection and their combination with enzymatic reactors enabling on-line digestion of (glyco)proteins and their subsequent analysis will be investigated. For HILIC method, studies of separation selectivity and retention mechanisms for analyses of porphyrin dyes in LC-MS mode will continue. In the field of dyes and pigments the research will be focused on the effects of production conditions on the pigments quality with emphasis on the determination of residual impurities. There will be developed analytical method for evaluation of mead quality by separation techniques (HPLC, CZE) and markers suitable for mead quality assessment. Development of LC-MS methods will continue for analysis of glycosphingolipids and eicosanoids. Final methods will be used for biological sample analyses in order to search differences among healthy volunteers and patients with cancer diseases. Obtained results will be evaluated by multidimensional statistical methods in order to find lipids with most increased and/or decreased expression due to the influence of illness studied. Modern microextraction approaches for aromaprofile analysis of beer and beer raw materials followed by analysis using GC-FID/MS methods will be evaluated. Studies focused on the analysis of volatile compounds in plant materials using various extraction methods (SPME, SFE, etc.) with subsequent separation by GC-MS will continue. Possibilities of analysis of post-explosion combustion products from improvised explosives will be investigated using modern GC-MS methods and also analyses of amines after their derivatisation will be realised by HPLC with fluorescence detection. Effect of drying temperatures (40-70 °C) on the buckwheat crackers quality will by studied. Development of cream cheese with functional properties will be implemented. In the field of electrochemical research the modification of carbon electrodes by ?-cyclodextrin will be used for determination of selected phenolic compounds in foodstuffs. Applications of carbon paste and printed electrodes for determination of selected alkaloids as well as extractive stripping voltammetry on paste electrodes for determination of milk lipophilic vitamins will continue. Enzymatic biosensors for monitoring of significant phenolic compounds will be developed. There will also continue the development of novel ion-selective types of electrodes. Modifications of microelectrode detectors for HPLC without necessity of reference electrode use will be tested. In the field of isotachophoresis the research will be focused on the reactions of sulphites with aldehydes and on the isotachophoretic determination of amino acids. The main aims on the case of atomic spectrometry will be the development and optimisation of effective methods for determinations, which are generally considered to be problematic and/or nearly impracticable in analytical practice. Research will be focused on the analysis of foodstuffs, biological, environmental and industrial samples.
New capillary monolithic column will be prepared and their chromatographic properties studied in order to investigate their possible utilisation for separation of phenolic compounds and flavones, nucleosides and other compounds in systems based on the HILIC and RP retention mechanisms. Research of the physico-chemical properties determination of micellar systems will continue. In this case, various methods for determination of critical micellar concentration of sodium alkylsulphates and volatile perfluorinated acids as surfactants suitable for connection with mass spectrometry will be studied. Application of two-dimensional separation techniques such as capillary chromatography and capillary electrophoresis followed by fluorescence detection and their combination with enzymatic reactors enabling on-line digestion of (glyco)proteins and their subsequent analysis will be investigated. For HILIC method, studies of separation selectivity and retention mechanisms for analyses of porphyrin dyes in LC-MS mode will continue. In the field of dyes and pigments the research will be focused on the effects of production conditions on the pigments quality with emphasis on the determination of residual impurities. There will be developed analytical method for evaluation of mead quality by separation techniques (HPLC, CZE) and markers suitable for mead quality assessment. Development of LC-MS methods will continue for analysis of glycosphingolipids and eicosanoids. Final methods will be used for biological sample analyses in order to search differences among healthy volunteers and patients with cancer diseases. Obtained results will be evaluated by multidimensional statistical methods in order to find lipids with most increased and/or decreased expression due to the influence of illness studied. Modern microextraction approaches for aromaprofile analysis of beer and beer raw materials followed by analysis using GC-FID/MS methods will be evaluated. Studies focused on the analysis of volatile compounds in plant materials using various extraction methods (SPME, SFE, etc.) with subsequent separation by GC-MS will continue. Possibilities of analysis of post-explosion combustion products from improvised explosives will be investigated using modern GC-MS methods and also analyses of amines after their derivatisation will be realised by HPLC with fluorescence detection. Effect of drying temperatures (40-70 °C) on the buckwheat crackers quality will by studied. Development of cream cheese with functional properties will be implemented. In the field of electrochemical research the modification of carbon electrodes by ?-cyclodextrin will be used for determination of selected phenolic compounds in foodstuffs. Applications of carbon paste and printed electrodes for determination of selected alkaloids as well as extractive stripping voltammetry on paste electrodes for determination of milk lipophilic vitamins will continue. Enzymatic biosensors for monitoring of significant phenolic compounds will be developed. There will also continue the development of novel ion-selective types of electrodes. Modifications of microelectrode detectors for HPLC without necessity of reference electrode use will be tested. In the field of isotachophoresis the research will be focused on the reactions of sulphites with aldehydes and on the isotachophoretic determination of amino acids. The main aims on the case of atomic spectrometry will be the development and optimisation of effective methods for determinations, which are generally considered to be problematic and/or nearly impracticable in analytical practice. Research will be focused on the analysis of foodstuffs, biological, environmental and industrial samples.
