Modern instrumental methods in analysis of materials, food and biological samples
Provider: Univerzita Pardubice
Programme: Studentská grantová soutěž
Implementation period: 01.01.18 - 31.12.18
Workplace:
Fakulta chemicko-technologická - FCHT
Investigator: Ventura KarelTeam member: Adam Martin | Červenka Libor | Fischer Jan | Holčapek Michal | Česlová Lenka | Meloun Milan | Švancara Ivan | Česla Petr | Bajerová Petra
Description:
Two-dimensional separation research will focus on increasing the sensitivity of the technique by using the focusing of analytes to transfer fractions between separating columns. Electromigration methods and methods of liquid chromatography will be developed for analyses of significant natural substances, industrial products and pharmaceuticals. The development and validation of methods for the analysis of eicosanoids and gangliosides will continue. Analyses of large series of samples of body fluids of patients with various types of cancer, healthy volunteers and blinded samples will continue. The data obtained will be statistically treated. Modern micro-extraction techniques for the analysis of hop profile and compounds characteristic of cold-brewed beers will be studied. The kinetics of sulphur compounds will be studied after breaking the onion and garlic tissue. The differences between the silicas obtained by different methods of isolation from different plant materials will be observed. Analyses of hydrosols resulting from the distillation of silicas from various plant materials will be carried out. Methods will be developed to analyse volatile compounds emitted from different types of wood. The possibilities of analysing explosion-induced explosives using chromatographic methods will be explored. Attention will be paid to the optimization of extraction and separation techniques for the analysis of biologically important substances contained in natural matrices. Above all, it will be about monitoring the content of phenolic substances in selected cereals and pseudo-bovines. Phenolic substances and other substances with antioxidant effect in olive oil will be analysed. Studies on the analysis of amino acids and phenolic acids in meadows will be completed. Cooperation with industry will be continued in the field of dye and pigment analysis. Significant volatile and non-volatile substances in green tea and coffee will be monitored. Research into new types of electrodes based on carbon and other non-mercury materials for the voltammetric study of biologically active substances and in the field of multi-channel separation systems with electrochemical detection will be continued. New types of biosensors will be prepared. Ion-selective electrodes will be applied to determine new types of non-ionic surfactants. Procedures for isotachophoretic determination of selected compounds will be developed. The study of the influence of the technological conditions of buckwheat build-up on the content of selected phenolic substances determined by the HPLC method will be continued. The effect of grape seed additions on the antioxidant and rheological properties of melted cheese will be monitored. The addition of roasted and unprocessed carob flour to the bakery recipe will be tested with regard to the resulting functional properties. In the field of atomic spectrometry, the main objective will be the development and optimization of new methods for the needs of inorganic elemental analysis of environmental samples, biological materials, food, feed and industrial samples. For eight new drugs mainly antitumor cytostatics, immunosupressives and antidepressives a research will be carried out to determine necessary physico-chemical constants and analytical properties such as solubility, dissociation constants, spectra and distribution diagrams of variously protonated species in protonation equilibria of the newest medicaments. Advance multivariate statistical data analysis will be applied on various biochemical and instrumental data to build an actual model and to predict hidden properties in biochemistry and medicine.
Two-dimensional separation research will focus on increasing the sensitivity of the technique by using the focusing of analytes to transfer fractions between separating columns. Electromigration methods and methods of liquid chromatography will be developed for analyses of significant natural substances, industrial products and pharmaceuticals. The development and validation of methods for the analysis of eicosanoids and gangliosides will continue. Analyses of large series of samples of body fluids of patients with various types of cancer, healthy volunteers and blinded samples will continue. The data obtained will be statistically treated. Modern micro-extraction techniques for the analysis of hop profile and compounds characteristic of cold-brewed beers will be studied. The kinetics of sulphur compounds will be studied after breaking the onion and garlic tissue. The differences between the silicas obtained by different methods of isolation from different plant materials will be observed. Analyses of hydrosols resulting from the distillation of silicas from various plant materials will be carried out. Methods will be developed to analyse volatile compounds emitted from different types of wood. The possibilities of analysing explosion-induced explosives using chromatographic methods will be explored. Attention will be paid to the optimization of extraction and separation techniques for the analysis of biologically important substances contained in natural matrices. Above all, it will be about monitoring the content of phenolic substances in selected cereals and pseudo-bovines. Phenolic substances and other substances with antioxidant effect in olive oil will be analysed. Studies on the analysis of amino acids and phenolic acids in meadows will be completed. Cooperation with industry will be continued in the field of dye and pigment analysis. Significant volatile and non-volatile substances in green tea and coffee will be monitored. Research into new types of electrodes based on carbon and other non-mercury materials for the voltammetric study of biologically active substances and in the field of multi-channel separation systems with electrochemical detection will be continued. New types of biosensors will be prepared. Ion-selective electrodes will be applied to determine new types of non-ionic surfactants. Procedures for isotachophoretic determination of selected compounds will be developed. The study of the influence of the technological conditions of buckwheat build-up on the content of selected phenolic substances determined by the HPLC method will be continued. The effect of grape seed additions on the antioxidant and rheological properties of melted cheese will be monitored. The addition of roasted and unprocessed carob flour to the bakery recipe will be tested with regard to the resulting functional properties. In the field of atomic spectrometry, the main objective will be the development and optimization of new methods for the needs of inorganic elemental analysis of environmental samples, biological materials, food, feed and industrial samples. For eight new drugs mainly antitumor cytostatics, immunosupressives and antidepressives a research will be carried out to determine necessary physico-chemical constants and analytical properties such as solubility, dissociation constants, spectra and distribution diagrams of variously protonated species in protonation equilibria of the newest medicaments. Advance multivariate statistical data analysis will be applied on various biochemical and instrumental data to build an actual model and to predict hidden properties in biochemistry and medicine.
