Multidimensional chromatography - mass spectrometry quantitative workflows in detailed characterization of human plasma lipidome
Provider: Grantová agentura ČR
Programme: Standardní projekty
Implementation period: 01.01.21 - 31.12.23
Workplace:
Fakulta chemicko-technologická - Katedra analytické chemie
Investigator: Holčapek Michal
Description:
Lipids are important biomolecules with various functions in cell membranes, signaling, and energy storage, which exhibit a huge structural diversity in the nature. The dysregulation of lipids can directly reflect various pathological states of human organism. Here we plan to develop a new strategy for the lipidomic quantitation of human plasma using two complementary approaches based on 13C stable isotope labeling, such as internal standards biosynthetized from human cell lines in labeled substrate and chemical derivatization with labeled agents. Labeling approaches will be implemented in a novel configuration of multidimensional analytical system, which should provide the most comprehensive characterization of human plasma lipidome ever reported for over one thousand lipids. The key expected benefit is the improvement of the comparability of results among different analytical laboratories and platforms with possible application in biomarker discovery and precision medicine.
Lipids are important biomolecules with various functions in cell membranes, signaling, and energy storage, which exhibit a huge structural diversity in the nature. The dysregulation of lipids can directly reflect various pathological states of human organism. Here we plan to develop a new strategy for the lipidomic quantitation of human plasma using two complementary approaches based on 13C stable isotope labeling, such as internal standards biosynthetized from human cell lines in labeled substrate and chemical derivatization with labeled agents. Labeling approaches will be implemented in a novel configuration of multidimensional analytical system, which should provide the most comprehensive characterization of human plasma lipidome ever reported for over one thousand lipids. The key expected benefit is the improvement of the comparability of results among different analytical laboratories and platforms with possible application in biomarker discovery and precision medicine.
