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Observation of Antioxidative Activity of Uric Acid in Men
Authors: Kanďár Roman | Žáková Pavla | Malíková Jana | Čegan Alexander | Skalický Jiří
Year: 2003
Type of publication: ostatní - přednáška nebo poster
Name of source: Clinical Chemistry and Laboratory Medicine Special Supplement
Publisher name: Walter de Gruyter GmbH & Co. KG
Place: Berlin
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Language Name Abstract Keywords
cze Sledování antioxidační aktivity kyseliny močové u lidí uric acid, antioxidant, allantoin, HPLC, oxidative stress, patients with chronic renal failure
eng Observation of Antioxidative Activity of Uric Acid in Men Uric acid, the final product of human purine metabolism, can be oxidized by various reactive oxygen species to allantoin. Therefore, the allantoin concentration may correlate with the extent of oxidative stress in humans. Aim: To compare allantoin concentration in plasma and erythrocytes of the patients with oxidative stress and the control group to verify this hypothesis. Concentration of allantoin was measured after its derivatization to glyoxylate-2,4-DNPH in plasma and erythrocytes obtained from patients with chronic renal failure before hemodialysis (n = 30, 15 women and 15 men) and from group of blood donors (n = 30, 15 women and 15 men) using HPLC (reversed phase, with gadient elution and UV-VIS detection at 360 nm). We found the significant differences in allantoin concentration between the patients and the control group both in plasma (20.5 +- 6.5 micoM vs. 2.7 +- 0.7 microM; p < 0.001) and erythrocyres (82.8 +- 39.1 nmol/g Hb vs. 21.8 +- 6.1 nmol/g Hb; p < 0.001). Statistical significance was evaluated by Student's t-tes. P < 0.001 values were concidered statistically significant. Conclusion: Significantly higher levels of allantoin both in plasma and erythrocytes of patients with chronic renal failure indicate that allantoin may be used as a good marker of oxidative stress. This work supported by grants GACR 203/02/0023, MSMT 253 100 002. uric acid, antioxidant, allantoin, HPLC, oxidative stress, patients with chronic renal failure

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