Publication detail

Detection of aflatoxinogenic fungi in plants

Authors: Šnévajsová Petra | Vytřasová Jarmila | Brožková Iveta | Pejchalová Marcela

Year: 2008

Type of publication: článek ve sborníku

Name of source: Biotechnologie 2008

Publisher name: Scientific Pedagogical Publishing

Place: České Budějovice

Page from-to: 261-264

Titles:

Language	Name	Abstract	Keywords
cze	DETEKCE POTENCIÁLNĚ AFLATOXINOGENNÍCH PLÍSNÍ V ROSTLINÝCH MATERIÁLECH	Práce se zabývá detekcí plísní rodu Aspergillus, které jsou jedním z nejčastějších kontaminantů potravin. Zamoření potravin ale i krmiv jak plísněmi rodu Aspergillus, tak jejich toxickými metabolity je závažný problém, neboť mají nepříznivý účinek na zdraví lidí a živočichů.	aflatoxinogenní plísně, PCR, Aspergillus
eng	Detection of aflatoxinogenic fungi in plants	The study examined occurrence of fungi in food. 91 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. Two samples contained two species fungi of genus Aspergillus and one sample contained three species fungi of genus Aspergillus. Finally we determined 31 kinds of the potentially aflatoxinogenic fungi on the AFPA medium. The PCR were positive for 27 kinds of the aflatoxinogenic fungi. Isolated Aspergillus strains were examined using PCR method. Method was optimized by using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. Specific PCR product (apa-2 gene and ver-1 gene) codes the biosynthetic way of aflatoxin B1. PCR product was detected by electrophoresis.	aflatoxinogenic fungi, PCR, Aspergillus

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