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PCR assay for detection of Streptococcus dysgalactiae ssp. equisimilis
Authors: Lysková Petra | Vydržalová Markéta | Kroupa Tomáš | Mazurová Jaroslava
Year: 2008
Type of publication: článek ve sborníku
Name of source: Clinical Microbiology and Infection
Publisher name: Blackwell Publishing Ltd
Place: Oxford
Page from-to: S397
Titles:
Language Name Abstract Keywords
cze PCR detekce Streptococcus dysgalactiae ssp. equisimilis Streptococcus dysgalactiae ssp. equisimilis patří mezi streptokoky se skupinovým antigenem G, které tvoří heterogenní skupinu beta-hemolytických streptokoků. V současnosti se do této serologické skupiny řadí tři taxony: Streptococcus dysgalactiae ssp. equisimilis, S. canis a skupina S. anginosus. Mohou vyskytovat na kůži a na sliznicích nosohltanu, gastrointestinálního a urogenitálního traktu lidí. V poslední době jsou však stále častěji zjišťovány jako závažná příčina různých, převážně akutních infekcí, jako jsou endokarditidy, artritidy, faryngitidy a sepse. Většina klinických laboratoří neprovádí druhovou typizaci streptokoků, ale omezuje se pouze na posouzení morfologie kolonií, biochemických vlastností a průkaz skupinového antigenu. Cílem naší studie bylo vyvinout novou PCR metody, která by umožnila rychlou a spolehlivou identifikaci S. dysgalactiae ssp. equisimilis. streptokoky se skupinovým antigenem G,PCR,S. dysgalactiae ssp. equisimilis
eng PCR assay for detection of Streptococcus dysgalactiae ssp. equisimilis Objectives: Streptococcus dysgalactiae ssp. equisimilis belongs to Lancefield serogroup G, which forms heterogeneous group of beta-hemolytic streptococci. Presently, three taxonomic types of this serological group can be distinguished, as follows: minute colony formers from humans (S. anginosus group), and large colony formers from humans (S. dysgalactiae ssp. equisimilis) and animals (S. canis). In humans, they may colonize pharynx, skin, gastrointestinal and female genital tract. In recent years, they have been reported with increasing frequency as a cause of variety of human infections, such as endocarditis, arthritis, pharyngitis and sepsis. The differentiation of streptococci traditionally relies on the evaluation of growth, serological and biochemical properties. However, developments in genetic technology have resulted in new methods that can be used for identification of bacteria. The aim of the present work was the development of new PCR assay for the rapid and reliable identification of S. dysgalactiae ssp. equisimilis. Methods: Based on the sequence analysis of the 16S-23S rDNA intergenic spacer region of eight S. dysgalactiae ssp. equisimilis strains of human origin species-specific primers were designed using the MEGA program, version 3.1. Subsequently, a PCR with these primers was optimalized and was used to identify S. dysgalactiae ssp. equisimilis strains isolated from humans. Results: The oligonucleotide primers designed according to the species-specific parts of the 16S-23S rDNA intergenic spacer region yielded an amplicon with a size of 205 bp for all S. dysgalactiae ssp. equisimilis strains investigated. S. canis, also belonging to Lancefield?s serogroup G, and all the other control strains of various species and serogroups were negative throughout. The limit of detection of S. dysgalactiae ssp. equisimilis was determined to be 1,4 ng DNA. Conclusion: PCR method presented in this study allowed a rapid and reliable identification of S. dysgalac group G streptococci,PCR,S. dysgalactiae ssp. equisimilis

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