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Detection of Mycoplasma hominis in amniotic fluid of pregnant women by real-time PCR
Authors: Vydržalová Markéta | Lysková Petra | Kroupa Tomáš | Mazurová Jaroslava
Year: 2008
Type of publication: článek ve sborníku
Name of source: Clinical Microbiology and Infection
Publisher name: Blackwell Publishing Ltd
Place: Oxford
Page from-to: S581-S582
Titles:
Language Name Abstract Keywords
cze Detekce Mycoplasma hominis v plodové vodě těhotných žen metodou real-time PCR Infekce matky během těhotenství hraje velkou roli při postižení plodu a spontánních potratech. Jednou z nějčastěji se vyskytujících bakterií při intrauterinních infekcích je Mycoplasma hominis. Naše studie hodnotila vztah mezi výskytem Mycoplasma hominis v plodové vodě, průběhem těhotenství a zdravotním stavem novorozence. Mycoplasma hominis,real-time PCR,plodová voda
eng Detection of Mycoplasma hominis in amniotic fluid of pregnant women by real-time PCR Objectives: Maternal infection has long been known to play a major role in perinatal morbidity and mortality and suspected to be a cause of spontaneous abortion. Intrauterine infection may be largely responsible for preterm delivery. The most common microorganism involved in intrauterine infections is Mycoplasma hominis. Mycoplasma hominis is commonly isolated microorganism from the female genital tract. It has been linked to pelvic inflammatory diseases such as salpingitis, endometritis, bacterial vaginosis and infertility. The transmition from the mother to the developing fetus can be in utero or at delivery. The relationship between detection of Mycoplasma hominis in mid-trimester amniotic fluid and subsequent pregnancy outcome was investigated. Data collected included indication for amniocentesis, gestational age at amniocentesis, gestational age at delivery, pregnancy outcome and low birth weight of infants. Method: The amniotic fluids of 202 women who underwent a transabdominal amniocentesis at weeks 15 - 22 of pregnancy were tested for Mycoplasma hominis by real-time PCR. Results: Mycoplasma hominis were identified in 51 (25.2 %) samples of the amniotic fluid. A total of two (28.6 %) infants, positive for Mycoplasma hominis, had the birth weight below 2500g. Ten of the women had preterm delivery and one of them (10 %) was positive for Mycoplasma hominis. Conclusion: In this study has not been found statistical correlation between Mycoplasma hominis and low birth weight and preterm delivery. Despite the detection of Mycoplasma hominis in samples of pregnant women should be considered. Real-time PCR is suitable for diagnostic of this microorganism in samples of amniotic fluid. Mycoplasma hominis,real-time PCR,amniotic fluid

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