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Comparison of High-fat Nutritional Models of Non-alcoholic Fatty Liver Disease in Rats.
Authors: Kučera Otto | Roušar Tomáš | Lotková Halka | Křiváková Pavla | Garnol Tomáš | Gunčová Ivana | Červinková Zuzana
Year: 2008
Type of publication: článek ve sborníku
Name of source: Česká a slovenská gastroenterologie a hepatologie
Publisher name: Česká lékařská společnost J. E. Purkyně
Place: Praha
Page from-to: 101-102
Titles:
Language Name Abstract Keywords
cze Srovnání High-fat nutričních modelů pro studium NAFLD u krys Srovnání High-fat nutričních modelů pro studium NAFLD u krys
eng Comparison of High-fat Nutritional Models of Non-alcoholic Fatty Liver Disease in Rats. Nonalcoholic fatty liver disease (NAFLD) is an important cause of liver-related morbidity and mortality. NAFLD is often the hepatic manifestation of metabolic syndrome and represents a wide spectrum of conditions ranging from nonprogressive hepatic steatosis, to nonalcoholic steatohepatitis that may progress to cirrhosis and end-stage liver disease. The aim of this project is to establish, characterize and compare 4 nutritional models of NAFLD on rats. Most suitable model will be used for estimation of toxic effects of model hepatotoxins on intact liver and liver affected by NAFLD. Wistar or Sprague-Dowly male rats were fed ad libitum a standard pelleted diet (ST-1, 10 % of energy from fat); mediumfat gelled diet (MFGD, 35 % of energy from fat) and high-fat gelled diet (HFGD, 71 % of energy from fat) for 3 or 6 weeks. Then serum ALT, AST, glycaemia, levels of triacylglycerols and cholesterol were measured. Respiration of isolated liver mitochondria was assessed using high-resolution respirometry. Malondialdehyde content in the liver and tissue cytokines (TNFalpha, IL-6, TGF-beta) were measured and histopathological samples were prepared (H+E, Sudan III). Feeding with HFGD (less in MFGD) induced periportal smalldroplet steatosis in comparison with control group (ST-1). Changes were more pronounced in 6-week models and in Sprague-Dowly strain of rats, where mild focal inflammation appeared. There were no significant differences among groups in each model in serum biochemical parameters except lower concentrations of triacylglycerols in MFGD and HFGD against ST-1. We found a significant decrease in activity of respiratory complex I in MFGD and HFGD groups in Wistar rats and increase in tissue TNF-alpha and IL-6 in HFLD in comparison with ST-1. NAFLD, HFGD, steatohepatitis.

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