Publication detail

Electroimmunoassay for Detection of Bacterial Cells

Authors: Vytřasová Jarmila | Hrubeš Michal | Červenka Libor | Pešková Iva | Vytřas Karel

Year: 2008

Type of publication: ostatní - přednáška nebo poster

Name of source: Electrochemistry for a Healthy Planet

Publisher name: International Society of Electrochemistry

Place: Lausanne

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
cze	Elektroimunochemická detekce bakteriálních buněk	Cílem příspěvku je navrhnout ampérometrickou metodu pro detekci bakterií rodu Salmonella s využitím uhlíkových pastových elektrod.	imunochemická detekce, Salmonella, protilátky
eng	Electroimmunoassay for Detection of Bacterial Cells	As an example of electroimmunoassay of bacterial cells, an amperometric method for Salmonella detection is presented. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase) forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals are monitored at 0.65 V vs. Ag/AgCl/satd. KCl in a medium of pH 10.0 which was found as the best. Amount of phenol generated by SAAP is proportional to the number of Salmonella Enteritidis in a sample.	Electroimmunoassay, Salmonella, Antibodies

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