Publication detail

Detection of Aflatoxinogenic Fungi Using PCR Method

Authors: Šnévajsová Petra | Brožková Iveta | Vytřasová Jarmila | Pejchalová Marcela

Year: 2008

Type of publication: ostatní - přednáška nebo poster

Name of source: 21st International ICFMH Symposium: Evolving microbial food quality and safety

Publisher name: University of Aberdeen Central Printing Service

Place: Aberdeen

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
cze	Detekce aflatoxinogenních plísní pomocí PCR	Aflatoxiny jsou sekundární metabolity A. flavus, A. parasiticus, A. nomius a A. tamarii. Biosyntetická dráha je kódována geny apa-2 a ver-1.	aflatoxiny, Aspergillus, PCR
eng	Detection of Aflatoxinogenic Fungi Using PCR Method	Aflatoxins are produced as secondary metabolites by A. flavus, A. parasiticus, A. nomius and A. tamarii. The aflatoxin biosynthetic pathway involves several enzymatic steps and genes (apa-2, ver-1) that appear to be regulated by the aflR gene in these fungi. The aim of this work was the optimization of the polymerase chain reaction applied to two genes encoding the biosynthesis of aflatoxins (apa-2 a ver-1, aflR), the verification of the reaction specific character, and the use of the method to speed up the confirmation of the presence of aflatoxigenic fungi isolated from food and feed.	aflatoxins, Aspergillus, PCR

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