Publication detail

Detection of Aflatoxinogenic Fungi Using PCR Method

Authors: Zachová Iveta | Vytřasová Jarmila | Pejchalová Marcela | Červenka Libor | Tavčar-Kalcher Gabrijela

Year: 2003

Type of publication: ostatní - přednáška nebo poster

Name of source: 1st FEMS Congress of European Microbiologists

Publisher name: Elsevier Sci

Place:

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Titles:

Language	Name	Abstract	Keywords
cze	detekce aflatoxinogenních plísní pomocí PCR metody		Aflatoxinogenic fungi; PCR; Aspergillus parasiticus, Aspergillus flavus
eng	Detection of Aflatoxinogenic Fungi Using PCR Method	: This work covers the possibility of using PCR method for acceleration and more accurate identification of the aflatoxinogenic fungi isolated from feed and foodstuffs. The method was optimalized on pure cultures (Aspergillus flavus CCM F-108 and Aspergillus parasiticus CCM F/550). The specificity of the optimalized PCR method was verified using various fungal strains. 50 samples of feed were examined, of which 18 were positive for the presence of the aflatoxinogenic fungi on AFPA medium. Isolated Aspergillus strains were examined using PCR method. The results obtained almost always agreed with the results gained on the AFPA medium. This method is a possible starting point for accelerating the detection of the aflatoxinogenic fungi, but it will be necessary to solve certain non-specific reactions, which are caused by a complex sample matrix. The results obtained are an initial step for further research. The PCR technique has proved itself in the detection of aflatoxinogenic fungi isolated from feed.	Aflatoxinogenic fungi; PCR; Aspergillus parasiticus, Aspergillus flavus

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