Publication detail

Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from Meat-Processing Plants by a Culture Technique and Detection by PCR

Authors: Vytřasová Jarmila | Pejchalová Marcela | Harsová Klára | Bínová Šárka

Year: 2003

Type of publication: článek v odborném periodiku

Name of source: Folia Microbiologica

Publisher name: Springer

Page from-to: 227-232

Titles:

Language	Name	Abstract	Keywords
cze	Izolace Arcobacter butzleri a A. cryaerophilus ve vzorcích masa metodou PCR	Izolace Arcobacter butzleri a A. cryaerophilus ve vzorcích masa metodou PCR	Arcobacter butzleri;Arcobacter cryaerophilus;PCR
eng	Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from Meat-Processing Plants by a Culture Technique and Detection by PCR	A pilot survey of sources of contamination with arcobacters, which represent a potential risk for humans, is described. A wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.), both from a retail level and domestic farming. Sanitary practices in abattoirs and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The applied method was based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical for the genus Arcobacter, and a product of 686 bp, specific for A. butzleri. On the whole, a total number of 198 samples was tested, of that 33 (16.7%) were found to be positive for genus Arcobacter but only 22 (11.1%) for A. butzleri.	Arcobacter butzleri;Arcobacter cryaerophilus;PCR

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