Publication detail

Study of acetaminophen hepatotoxicity in vitro - role of glutathione reductase inhibition.

Year: 2009

Type of publication: ostatní - článek ve sborníku

Name of source: Proceedings of the 27th annual Workshop of the Scandinavian Society for cell toxicology

Publisher name: Tribun EU

Place: Brno

Page from-to: 46-46

Titles:

Language	Name	Abstract	Keywords
cze	Studium acetaminofenové toxicity in vitro - role glutathionreduktasy	Studium acetaminofenové toxicity in vitro a role glutathionreduktasy během APAP toxicity byla vyštřována in vitro. byl nalezen velmi vysoký inhibiční efekt AG konjugátu a byla prokázána na dávce závislá inhibice. Tyto výsledky mohou vést k objevení nové cesty při léčbě APAP předávkování.
eng	Study of acetaminophen hepatotoxicity in vitro - role of glutathione reductase inhibition.	Acetaminophen (paracetamol, APAP) is one of the most used antipyretics and analgetics in present. It is a safe drug at therapeutical doses; however, the overdose may lead even to the acute liver failure. The aim of our work was to find if the glutathione reductase (GR) inhibition may contribute to the impairment of APAP treated cells. We used rat hepatocytes isolated by two step collagenase perfusion and cultivated on Petri dishes. The inhibitory effect was assessed in a solution where GR prepared from rat hepatocytes and the other reagents were present (NADPH, GSSG). We found an inhibitory effect in the presence of APAP-SG; we proved a decrease of GR activity by 30 % and 60 %, in 1.5 mM and 3.7 mM APAP-SG treated samples, respectively (compared to control). The described inhibitory effect of APAP-SG conjugate may play likely an important role in the mechanism of APAP toxicity due to deepening of the typical glutathione depletion in the hepatocytes.	Acetaminophen; hepatocytes in culture; hepatotoxicity.

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