Publication detail

The effect of thioacetamide on rat liver in vitro and in vivo.

Year: 2009

Type of publication: ostatní - článek ve sborníku

Name of source: Proceedings of the 27th annual Workshop of the Scandinavian Society for cell toxicology

Publisher name: Tribun EU

Place: Brno

Page from-to: 34-34

Titles:

Language	Name	Abstract	Keywords
cze	Studium toxicity thioacetamidu na potkaních játrech v podmínkách in vitro a in vivo	Studium toxicity thioacetamidu na játrech v podmínkách in vitro a in vivo bylo prováděno na potkanech. byl testován vliv na energetický metabolismus, oxidační pochody a viabilitu. byl zjištěn významný vliv na aktivity dých. řetězce a také na viabilitu buněk.
eng	The effect of thioacetamide on rat liver in vitro and in vivo.	Thioacetamide (TAA) is hepatotoxin and hepatocarcinogen frequently used for experimental purposes. Single dose of this hepatotoxin could produce centrilobular necrosis with subsequent regenerative response. The precise mechanism of its toxic effect is not fully understood. The aim of this work was to study the effect of TAA in vitro and after single dose in vivo. Our results indicate that TAA inhibits to a greater extent the oxidation of NADH-dependent respiratory substrates (70 mM TAA - 25%) than oxidation of flavoprotein-dependent substrate succinate (70 mM - 45%). The signs of regenerative response were observed after 48 hours. Mitochondrial respiration activated by NADH-dependent dehydrogenases was decreased only after 24 hours (70%) and the activity of succinate dehydrogenases was not influences in any time interval. It seems that modulation of substrate specificity could be interesting strategy to supply energy demand in regenerating liver.	Thioacetamide; hepatocytes in culture; hepatotoxicity; high resolution respirometry.

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