Publication detail

Model of acetaminophen-induced injury of rat hepatocytes in primary culture.

Authors: Kučera Otto | Roušar Tomáš | Staňková Pavla | Lotková Halka | Červinková Zuzana

Year: 2009

Type of publication: ostatní - článek ve sborníku

Name of source: Proceedings of the 27th annual Workshop of the Scandinavian Society for cell toxicology

Publisher name: Tribun EU

Place: Brno

Page from-to: 29-29

Titles:

Language	Name	Abstract	Keywords
cze	Zavedení modelu aceaminofenového poškození u kultivovaných potkaních hepatocytů	Zavedení modelu aceaminofenového poškození u kultivovaných potkaních hepatocytů
eng	Model of acetaminophen-induced injury of rat hepatocytes in primary culture.	Acetaminophen (APAP) overdose is the most common cause of drug-induced acute liver failure. While metabolic activation of APAP is an essential step in the development of toxic lesion, precise mechanisms of the action of APAP in hepatotoxicity remain to be elucidated. The aim of our study was to establish a model of APAP-induced toxic injury of rat hepatocytes in vitro. APAP caused damage to hepatocyte membrane and a decrease in activities of dehydrogenases in dose- and time- dependent manner. Production of albumin, GSH content in hepatocytes and number of cells resp. mitochondria with high MMP were in reciprocal proportion to the dose of APAP after 24 h. Production of MDA was increasing with the dose of APAP. ROS production reached the highest level at APAP concentration of 3.75 mM. APAP in the range of 2.5 to 5 mM and 24h incubation are optimal for model injury of hepatocytes in vitro.	Acetaminophen hepatotoxicity; hepatocytes in culture; oxidative stress.

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