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Využití CZE-LIF analýzy N-glykanů v klinické diagnostice nádorových onemocnění

Authors: Staněk Václav | Novotny Milos | Jandera Pavel

Year: 2009

Type of publication: ostatní - článek ve sborníku

Page from-to: 115-123

Titles:

Language	Name	Abstract	Keywords
cze	Využití CZE-LIF analýzy N-glykanů v klinické diagnostice nádorových onemocnění
eng		In present work, the applicability of capillary zone electrophoresis coupled with LIF detector as a powerful clinical diagnostic and prognostic tool for certain diseases resulting in the alteration of glycan profiles is demonstrated. Derivatized neutral and acidic N-linked glycans released from human glycoproteins from healthy and breast cancer patients were resolved using a polyacrylamide coated capillary with reduced electroosmotic flow. Glycan structure identification was elucidated using a combination of strategies, including comparison with standard glycan libraries and the enzymatic digestions of N-linked glycan pools using specific and non-specific exoglycosidases. By doing so, 47 glycan structures, many being structural isomers, were identified. Due to its ability to resolve structural isomers, CZE-LIF technique complements other means for glycan mapping such as a mass spectrometry.	CZE;N-glycans;Clinical diagnostics;Cancer