Publication detail

Utilization of the PCR method for detection of Arcobacter

Authors: Zachová Iveta | Vytřasová Jarmila | Husková Zuzana | Červenka Libor | Pejchalová Marcela

Year: 2004

Type of publication: ostatní - přednáška nebo poster

Name of source: 19 th International ICFMA Symposium - FOOD MICRO 2004

Publisher name: Slovenian Microbiological Society, Ljubljana

Place:

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Titles:

Language	Name	Abstract	Keywords
cze	Užití metody PCR pro detekci mikrobů rodu Arcobacter	určeno pro seznam publikací	Arcobacter butzleri, Arcobacter cryaerophilus, Polymerase Chain Reaction
eng	Utilization of the PCR method for detection of Arcobacter	The objective of this study was to accelerate the detection of Arcobacter spp. isolated from foodstuffs and water and observes an influence of samples´ matrix. Method of optimized multiplex PCR was verified on reference strains Arcobacter butzleri CCUG 30484, Arcobacter cryaerophilus CCM 3933 and Arcobacter cryaerophilus CCM 3934. Direct analysis of enrichment broths takeing-off a culture step was performed. Four enrichment media were tested for the acceleration of arcobacters´ detection. F6 selective broth yielded the best results. Poultry, porcine and bovine samples were artificially contaminated and the influnce of samples´ matrix was investigated. A choice combination of an enrichment broth and a plating agar was highly selective. Sorts of meat differ in a content of fat, which had an influence on a detection of arcobacters. 69 samples of food products and water were examined using multiplex PCR. Arcobacter spp. were found in 29 cases (42 %). Positive isolates were confirmed as A. butzleri (24,6 %) and A. cryaerophilus (17,4 %).	Arcobacter butzleri, Arcobacter cryaerophilus, Polymerase Chain Reaction

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