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Antibiotic resistance of staphylococci and enterococci isolated from water and foods
Authors: Vytřasová Jarmila | Zachová Iveta | Červenka Libor | Kolář Milan
Year: 2004
Type of publication: ostatní - přednáška nebo poster
Name of source: 19 th International ICFMA Symposium - FOOD MICRO 2004
Publisher name: Slovenian Microbiological Society, Ljubljana, Slovinsko
Place:
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Language Name Abstract Keywords
cze Antibiotická resistence stafylokoků a enterokoků izolovaných z vody a potravin určeno pro seznam publikací Staphylococci, Enterococci, antibiotics, minimum inhibitory concentration, vancomycin-resistant enterococci
eng Antibiotic resistance of staphylococci and enterococci isolated from water and foods The aim of this study was to find occurence of enterococci and staphylococci in foodstuffs and in water and to determine their resistance to antibiotics. Vancomycin-resistant enterococci represent a significant problem as they can produce a nosocomial infections. 260 samples of foodstuffs and water were examined, of which 105 were positive for the presence of enterococci and 67 of staphylococci. E. faecium (58,1%) and E. faecalis (34,3%) belong to the strains which were found most often. Other isolates were E. casseliflavus (3,3%) and E. mundti (0,95%). The most frequently isolated strains of staphylococci were S. warneri (25,4%), S. saprophyticus (16,4%) and S. epidermidis (10,4%). The isolated strains were identified and, based on their generic attributes, they were ranked to genus Enterococcus and Staphylococcus. For sorting, a Staphy test 16, API Staph and EN-COCCUS tests were used. The isolates were further subjected to screening of sensitivity to antibiotics, a microbroth dilution method was applied. It was observed that the tested strains of enterococci showed the highest sensitivity to teicoplanin (100%) and vancomycin (98,1%), five strains (4,8%) were resistant to both ampicillin and chloramphenicol. Concerning staphylococci, they were highly sensitive to teicoplanin and vancomycin (100%). Both benzylpenicillin and erythromycin appeared to be to least effective; percentages of staphylococci resistant to the first 63,5% and to the second antibiotic 44,9% were registered. Staphylococci, Enterococci, antibiotics, minimum inhibitory concentration, vancomycin-resistant enterococci