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Possibilities of measurement of malondialdehyde, a product of lipoperoxidation
Authors: Kanďár Roman | Žáková Pavla | Richter Michal
Year: 2004
Type of publication: ostatní - přednáška nebo poster
Name of source: Vitamins 2004 Targeted nutrional therapy
Publisher name: Univerzita Pardubice
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Language Name Abstract Keywords
cze Možnosti stanovení malondialdehydu, produktu lipoperoxidace malondialdehyd; oxidační stres; reaktivní sloučeniny kyslíku; lipoperoxidace; kapalinová chromatografie; 2-Thiobarbiturová kyselina
eng Possibilities of measurement of malondialdehyde, a product of lipoperoxidation The most widely adopted method for valuation of oxidative stress is the estimation of the level of malondialdehyde (MDA), a stable product of the oxidative degradation of polyunsaturated fatty acids with more than two double bonds. Usually the measurement of MDA is based on spectrophotometric or fluorimetric determination of its condensation product with 2‑thiobarbituric acid (TBA). Spectrometric determination of MDA level in the form of MDA‑TBA complex at 532 nm is a simple method with the sufficient detection limit. Unfortunately, the presence of interfering substances in crude plasma samples considerably decreases the specifity of this method. This is probably the reason for a rather wide range of values reported for MDA concentration in plasma samples by different authors. Esterbauer and co‑workers compared data on MDA concentration values in human plasma published by various authors. From 13 authors who used spectrophotometric TBA method, six obtained values in the range (0.32 ? 1.88) uM, five in the range (3.3 ? 4.6) uM, and two obtained even values of 47.2 uM and 35.1 uM. The specifity of analysis may be improved by simultaneous measurement of absorbance of the analyte at three wavelengths with Allen?s correction or by separation of the MDA‑TBA complex from interfering substances with HPLC. The addition of chelating agent (e.g. EDTA) to the sample is necessary to prevent further lipoperoxidation by metal ions. At present we develop HPLC method for the simultaneous measurement of MDA, formaldehyde, acetaldehyde, acetone, and propionaldehyde, products of lipid peroxidation. malondialdehyde; oxidative stress; reactive oxygen species; lipid peroxidation; liquid chromatography; 2-Thiobarbituric acid

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