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Výskyt toxinogenních plísní rodu Fusarium v potravinách
Authors: Brožková Iveta | Vytřasová Jarmila | Svobodová Lucie | Pejchalová Marcela | Červenka Libor
Year: 2006
Type of publication: článek ve sborníku
Name of source: Sborník Mikrobiologie potravin
Publisher name: Vysoká škola chemicko-technologická v Praze
Place: Praha
Page from-to: 93-98
Titles:
Language Name Abstract Keywords
cze Výskyt toxinogenních plísní rodu Fusarium v potravinách PCR procedure according to Edwards et al. (2001) was optimised with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F?683 and selectivity was examined using reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non?toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2?3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium.
eng Výskyt toxinogenních plísní rodu Fusarium v potravinách PCR procedure according to Edwards et al. (2001) was optimised with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F?683 and selectivity was examined using reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non?toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2?3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium. Fusarium, PCR, DNA isolation

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