Publication detail

Non-specific reactions in rapid detection of Listeria in foodstuffs using immunomagnetic separation (IMS)

Year: 2005

Type of publication: ostatní - přednáška nebo poster

Name of source: Book of abstracts, BioMicro World2005

Publisher name: Formatex Research Center

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Language	Name	Abstract	Keywords
cze	Rychlá detekce listerií v potravinách pomocí IMS a její nespecifické reakce	určeno pro seznam publikací	Listeria; imunomagnetická separace; protilátky
eng	Non-specific reactions in rapid detection of Listeria in foodstuffs using immunomagnetic separation (IMS)	Bacteria of genus Listeria are widely distributed in nature and due to environmental contamination, they can often be found in food. Listeria monocytogenes is an invasive pathogen, which attacks and multiplies inside of immune system cells. It causes a seldom but serious infections known as listeriosis. Detection of L. monocytogenes in foodstuffs by conventional cultivation methods carried out according to ISO 10560 for milk and dairy products [1] or according to the horizontal method EN ISO 11290-1 [2] is rather time-consuming and takes 4-6 days. Polymerase chain reactions (PCR) represent a feasible method for rapid detection of Listeria especially in connection with immunomagnetic separation (IMS). The use of IMS with Dynabeads â anti-Listeria (instead of the selective enrichment in Fraser broth) and application of PCR to distinguish L. monocytogenes among others Listeria reduced the time of analysis to 78 h [3]. Recently, problems of IMS as a tool of sample preparation method for direct detection of L. monocytogenes in cheese were published. The effect of the presence of non- Listeria flora on the sensitivity of the IMS method was also described [4]. The aim of this study was to examine non-specific reactions during IMS of Listeria and to compare different immunomagnetic particles. The pure cultures used were isolated from ripened soft cheese "Olomoucké tvarůžky". Gram-positive microorganisms, especially St. aureus and Arthrobacter spp., were found to be responsible for non-specific reactions in the most cases. The capacity of Dynabeads â anti-Listeria was determined to be about 10% only using pure cultures of Listeria. The influence of fat content was also monitored; samples of milk and whipped cream were examined. Non-specific reactions occurring during IMS of Listeria were examined on the artificially inoculated food samples in which gram-positive bacteria showed the highest percentage of capture. Food samples were investigated o	Listeria; Listeria monocytogenes; immunomagnetic separation; antibodies

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