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Prevalence and diversity of Arcobacter spp. in the Czech Republic
Authors: Pejchalová Marcela | Dostalíková Eva | Slámová Michala | Brožková Iveta | Vytřasová Jarmila
Year: 2008
Type of publication: článek v odborném periodiku
Name of source: Journal of Food Protection
Publisher name: International Association for Food Protection
Place: Des Moines
Page from-to: 719-727
Titles:
Language Name Abstract Keywords
cze Výskyt a diversita Arcobacter spp. v České Republice Výskyt a diversita Arcobacter spp. v České Republice arkobaktery; PCR; matrice
eng Prevalence and diversity of Arcobacter spp. in the Czech Republic The aim of this study was to examine 634 samples of chicken, lamb, pork, beef, fish, samples from the intensive animal industry and from poultry for slaughter as well as from the domestic breeding of poultry, horses, pigs, and lambs, surface water, and clinical samples for the presence of Arcobacter. All the samples were examined with a cultivation method followed by the confirmation of multiplex PCR. The method of multiplex PCR applied directly to a liquid medium after enrichment was applied only to the samples with the highest probability of the presence of arcobacters. Arcobacter spp. was detected in 11.8% of the samples, of which A. butzleri, A. cryaerophilus, and A. skirrowii was found in 6.6%, 5.1% and 0.2 % of the samples, respectively. The sources of the arcobacters were the chicken meat from the retail market, intensive animal industry, domestic breeding of chickens, lamb raising environment, surface water, and wastewater, and the swabs of beef taken in a meat processing factory. No occurrence of arcobacters was identified in the swabs from slaughter turkeys, ducks and wild poultry. No arcobacters were found in horse and pig breading environments, in pork, or in the swabs of fish. Forty-two rectal swabs taken from humans were also free of the bacteria of the genus Arcobacter. 17 isolates of Arcobacter were further identified by sequencing the 16S rRNA gene. Varied genotypes were observed among A. butzleri from chicken meat and chicken breeds and A. cryaerophilus from wastewater and chicken breeds,. They were similar to the genotypes present in wastewater, porcine feces, human stool and human blood obtained from databases. Our results revealed that the chicken meat from the retail market was an important source of arcobacters. Cross-contamination during handling practices could play a key role in the contamination of chicken carcasses. Arcobacter; PCR; matrix of sample

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