Publication detail

Různé metodiky izolace DNA aflatoxinogenních plísní

Authors: Šnévajsová Petra | Brožková Iveta | Vytřasová Jarmila

Year: 2007

Type of publication: článek ve sborníku

Name of source: Mikrobiologie potravin

Publisher name: Vysoká škola chemicko-technologická v Praze

Place: Praha

Page from-to: 99-106

Titles:

Language	Name	Abstract	Keywords
cze	Různé metodiky izolace DNA aflatoxinogenních plísní	V příspěvku je popsána optimalizace několika metod izolace plísňové DNA pro následnou PCR.	Aspergillus flavus; PCR; izolace DNA
eng	Comparison of various metod of isolation DNA from aflatoxinogenic fungi.	This work covers isolation of DNA from the toxinogenic fungi of genus Aspergillus. Filamntous fungi have strong cell walls which are often resistant to trditional method. DNA is extracted from fungal mycelium by a number methods. We used method relied on the mechanical disruption. Classical technique od DNA extraction was optimised and compared with use of commercial PCR kits.	Aspergillus flavus; PCR; DNA isolation

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