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Detekce mikroorganismů vyskytujícím se v životním prostředí pomocí molekulárně biologických metod
Authors: Pejchalová Marcela | Vytřasová Jarmila | Brožková Iveta
Year: 2006
Type of publication: článek ve sborníku
Name of source: Sborník semináře Monitorování cizorodých látek v životním prostředí
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 169-174
Titles:
Language Name Abstract Keywords
cze Detekce mikroorganismů vyskytujícím se v životním prostředí pomocí molekulárně biologických metod The polymerase chain reaction (PCR) belong to basic molecular biology techniques used in microbiological laboratory. Detection of large series of samples is provided by biochips and fluorescence probes are used in systems with fluorometric detection of amplified PCR products. The methods are highly sensitive, specific and rapid. Detection of patogenic bacteria is based on virulence genes identification. Escherichia coli, coliform bacteria, Clostridium perfringens,, Listeria monocytogenes, Vibrio cholereae, Vibrio parahemolyticus, Salmonella, Shigella, Campylobacter, Legionella and Giardia are most frequently monitored microorganisms in food samples, water samples as well as environmental samples.
eng Occurence and detection of microorganisms in environment using molecularbiological methods. The polymerase chain reaction (PCR) belong to basic molecular biology techniques used in microbiological laboratory. Detection of large series of samples is provided by biochips and fluorescence probes are used in systems with fluorometric detection of amplified PCR products. The methods are highly sensitive, specific and rapid. Detection of patogenic bacteria is based on virulence genes identification. Escherichia coli, coliform bacteria, Clostridium perfringens,, Listeria monocytogenes, Vibrio cholereae, Vibrio parahemolyticus, Salmonella, Shigella, Campylobacter, Legionella and Giardia are most frequently monitored microorganisms in food samples, water samples as well as environmental samples. PCR; pathogenic bacteria; virulence genes