Publication detail

Comparison of aflatoxinogenic fungal DNA isolation for PCR.

Authors: Šnévajsová Petra | Vytřasová Jarmila | Brožková Iveta | Pejchalová Marcela

Year: 2006

Type of publication: článek ve sborníku

Name of source: The Proceedings: FoodMicro 2006, Food safety and food biotechnology: diversity and global impact

Publisher name: Alma Mater Studiorum, University of Bologna

Place: Bologna

Page from-to: 453

Titles:

Language	Name	Abstract	Keywords
cze	Porovnání jednotlivých metod izolace DNA z aflatoxinogenních plísní.	určeno pro seznam publikací	aflatoxinogenní plísně, DNA izolace, PCR
eng	Comparison of aflatoxinogenic fungal DNA isolation for PCR.	Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol?ES. Methods were optimized by using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration. The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium.	aflatoxigenic fungi, DNA isolation, PCR

Search