Login for students

Login for employees

Publication detail

Aflatoxigenic fungi DNA isolation for preparation of DNA chip.
Authors: Šnévajsová Petra | Brožková Iveta | Vytřasová Jarmila
Year: 2006
Type of publication: ostatní - přednáška nebo poster
Name of source:
Publisher name:
Place:
Page from-to:
Titles:
Language Name Abstract Keywords
cze Izolace DNA pro detekci aflatoxinogenních plísní pomocí DNA čipu. určeno pro seznam publikací
eng Aflatoxigenic fungi DNA isolation for preparation of DNA chip. This work covers possibilities of DNA isolation and immobilization from the toxinogenic fungi of genus Aspergillus for preparation of DNA chip. Filamentous fungi have a sturdy cell wall. The most difficult step in the isolation of DNA from Aspergillus species is to disrupt cell wall without causing damage to genomic DNA. Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. Classical techniques included DNA extraction by Cenis (1992) and modified procedure by Shapira et al. (1996) in connection with Cenis (1992). Fungi mycelium was disrupted by ultrasonic bath and liquid nitrogen. Furthermore three commercial kits for DNA isolation from plant leaves, seeds and plants were used. For DNA isolation was also used commercially delivered compound DNAzol?ES. DNA was purified by immobilization on superparamagnetic silica particles and diatom. Methods were optimized by using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. Acquired DNA was amplified by PCR. Specific PCR product (apa-2 gene) was detected by electrophoresis. Records were interpreted. Purity and concentration of DNA were identified by spectrophotometer. Modified procedure by Shapira et al. (1996) in which liquid nitrogen is used to disrupt cell wall has proved as very effective. The PCR product coding biosynthetic way of aflatoxin B1 will use for construction of DNA chip. DNA chip, isolation, A.flavus

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement
CC BY-NC-ND 4.0