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Possibilities in the use of BacT/Alert SA bottles for diagnosing vascular catheter infections
Authors: Čermák Pavel | Ulrychová M | Víchová P | Mazurová Jaroslava
Year: 2006
Type of publication: článek ve sborníku
Name of source: Clinical Microbiology and Infection
Publisher name: Blackwell Publishing Ltd
Place: Oxford
Page from-to: R2067
Titles:
Language Name Abstract Keywords
cze Možnosti využití BacT/Alert SA v diagnostice infekcí vaskulárních katetrů Určeno pro seznam publikací
eng Possibilities in the use of BacT/Alert SA bottles for diagnosing vascular catheter infections Objectives: Infection caused by the insertion of vascular catheters is responsible for a high percentage of nosocomial infections. Catheter infections cause increased morbidity and mortality along with the raising cost of treatment. The new method is based on the transport of bacteria from catheter surface into solution and cultivation of this solution Bact/Alet system. Bacteria quantity present on the catheter is determined on the base of isolated agent and detection time (TTD). The TTD of positive vials is proportional to quantity of inoculated bacteria and their metabolic activity. Methods: 4 ml of sterile physiological solution was added to a test-tube containing sample of vascular catheter and the tube was agitated for 2 minutes. The whole volume of the solution from the tube with vascular catheter was inoculated into the bottle BacT/Alert SA. Bacteria quantity was determined by substitution TTD to the equation of TTD dependence on the number of inoculated CFU for separate groups of species. Results: In total 187 vascular catheters were elaborated. After seven days of cultivation 10 catheters were negative (5.34%). At the evaluating criterion 103 and more detected bacteria, 18 catheters were positive (9.62%). With respect to the correlation with clinical conditions 2 false positive results (1.06%) and 12 false negative results (6.41%) were found. On the basis of clinical conditions 28 (13.46%) cases of catheter sepsis in the set of 187 examined patients were diagnosed. Agreement of quantitative catheter examination with clinical finding occurred in 179 cases (92.7%). Coincidence of positive findings on the catheter with positive evaluation of clinical condition was seen in 16 cases (57.14%). The most frequent detected pathogens were coagulase negative staphylococci. Conclusion: The method is applicable in clinical practice and is able to detect most frequent agents of catheter infections. Number of bacteria caught on the surface of a catheter is a si BacT/Alert SA, vascular catheter, infections

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