Publication detail

Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro

Year: 2009

Type of publication: článek v odborném periodiku

Name of source: Physiological Research

Publisher name: Fyziologický ústav AV ČR

Place: Praha

Page from-to: 239-246

Titles:

Language	Name	Abstract	Keywords
cze	Hodnocení oxidačního stresu u kultivovaných potkaních hepatocytů inkubovaných s AAP	Práce popisuje AAP toxicitu na kultivovaných hepatocytech. K testování AAP toxicity byly použity AAP koncentrace: 1, 2.5, 5, 10 a 20 mM. V daných intervalech byla testována viabilita buněk, funkční kapacita a oxidační status. WST-1 test ukázal snížení dehydrogenasové aktivity u 5, 10 a 20 mM AAP na 75% kontrolního signálu už po 1 hod. inkubace. Funkční kapacita byla testována pomocí albumin-assay, kdy pokles byl jasně závislý ma dávce AAP. Intracelulární oxidační status byl vyšetřován pomocí stanovení GSH/GSSG hladin, ROS produkce a glutathionreduktasové (GR) aktivity. Zvýšení ROS produkce bylo nalezeno už po 3 h inkubace u 2.5, 5, 10 a 20 mM AAP. ROS produkce byla nejvyšší po 12 h . GR aktivita byla snížená po 3 h inkubace a přetrvala snížená u 2.5, 5, 10 a 20 mM AAP dále
eng	Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro	The present study describes estimation of acetaminophen toxicity in cultured rat hepatocytes. We used different concentrations of AAP : 1, 2.5, 5, 10 and 20 mM, to test influence of AAP on cellular viability, functional capacity and oxidative status at given time intervals. WST-1 test showed decrease of dehydrogenase activity in 5, 10 and 20 mM AAP to 75% of control signal already after 1 hour of incubation. Functional capacity was tested by albumin assay where the decrease was strictly related to AAP dose. Intracellular oxidative status was assessed by analysis of GSH/GSSG levels and time course of ROS production and glutathione reductase (GR) activity. Increased ROS production was found already after 3 h of incubation in 2.5, 5, 10 and 20 mM AAP, respectively. The highest ROS production was measured after 12 h treatment. GR activity was decreased already after 3 h of incubation and remained also decreased in 2.5, 5, 10 and 20 mM AAP treated cells during further time of incubation.	Acetaminophen; oxidative stress; hepatocyte.

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