Publication detail

Study of acetaminophen hepatotoxicity – the toxic effect of acetaminophen-glutathione conjugate on mitochondrial respiration.

Year: 2010

Type of publication: ostatní - přednáška nebo poster

Name of source: Česká a slovenská gastroenterologie a hepatologie

Publisher name: Česká lékařská společnost J. E. Purkyně

Place: Praha

Page from-to: S27

Titles:

Language	Name	Abstract	Keywords
eng	Study of acetaminophen hepatotoxicity – the toxic effect of acetaminophen-glutathione conjugate on mitochondrial respiration.	Currently, we reported that APAP-SG is able to inhibit glutathione reductase, an enzyme that accounts for backward reduction of glutathione disulphide. In the present work, we aimed to follow these results so that we estimated an influence of specific acetaminophen metabolite, APAP-SG on mitochondrial respiration and ROS production. We found that APAP-SG conjugate possessed an inhibition effect on mitochondrial respiration in both complex I and II. APAP-SG (5 mM) decreased the rate of respiration to 26 % and 55 % in complex I and II, respectively. An inhibition effect on complex I activity was found even in 0.2 mM APAP-SG (to 57 % of control signal); however, the complex II activity was not decreased at 0.2 mM APAP-SG. To estimate the level of intramitochondrial ROS production we used DCFDA probe. We proved that ROS formation was enhanced in the presence of APAP-SG and succinate as a substrate: to 900 % and 350 % of control signal, in 5 mM and 1 mM APAP-SG, respectively.	acetaminophen; hepatotoxicity; mitochondrial; respiration; glutathione

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