Publication detail

Electrochemical Analysis of DNA: When, How and Why to Use Electroactive Labels and Indicators

Year: 2010

Type of publication: článek ve sborníku

Name of source: Sborník přednášek mezinárodní odborné konference XXX. Moderní elektrochemické metody

Publisher name: BEST Servis

Place: Ústí nad Labem

Page from-to: 40-44

Titles:

Language	Name	Abstract	Keywords
cze	Elektrochemická analýza DNA: Kdy, jak a proč použít elektroaktivní značky a indikátory	Různé redox aktivní a enzymatické značení DNA bylo použito pro elektrochemickou detekci poškození DNA, DNA hybridizace a pro detekci (minisekvenování) záměn jednoho nukleotidu v řetězci DNA.	Elektrochemie DNA; elektroaktivní značky; redoxní indikátory; stanovení s využitím enzymů; zesílení signálu; strukturní sondy
eng	Electrochemical Analysis of DNA: When, How and Why to Use Electroactive Labels and Indicators	DNA possesses intrinsic electrochemical activity due to the presence of electrochemically reducible or oxidizable nucleobases and exhibits characteristic, structure-sensitive adsorption/desorption behavior at mercury or amalgam electrodes. Thus, label-free electrochemical DNA sensing is in principle possible, and especially in connection with mercury-based or carbon electrodes the intrinsic DNA electroactivity has been utilized to detect DNA damage. In other applications, such as sequence-specific DNA sensing, typing of single-nucleotide polymorphisms (SNP) etc., utilization of electroactive labels has appeared advantageous. We present several examples of using redox-active or enzymatic DNA tags in the detection of DNA damage, DNA hybridization and minisequencing of the SNP.	DNA electrochemistry; Label-free; Electroactive labels; Redox indicators; Enzyme-linked assays; Signal amplification; Structure probes

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