Login for students

Login for employees

Publication detail

Optimization of Selectivity, Peak Capacity and Separation Time in Two-Dimensional HPLC by Matching Separation Systems and Adjusting Gradient Conditions in the Two Dimensions
Authors: Jandera Pavel | Hájek Tomáš | Česla Petr
Year: 2010
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Optimization of Selectivity, Peak Capacity and Separation Time in Two-Dimensional HPLC by Matching Separation Systems and Adjusting Gradient Conditions in the Two Dimensions The main objectives of two-dimensional (2D) LC-LC separation techniques are: 1/ significant increase in the peak capacity, i.e., in the number of sample compounds separated in a single run in complex samples with respect to single-dimensional separations, and 2/ possibilities of sample separation into distinct classes on the basis of structural similarities. In-line "comprehensive" LCxLC has experienced rapid development during the past years, mainly because it allows automatic performance and significantly increased number of compounds separated per the time unit in comparison to single-dimension HPLC separations. The operation conditions in the two dimensions should match to provide maximum twodimensional peak capacity in the time available for the analysis. The chemistry of the stationary phase and the composition of the mobile phase should be optimized to provide noncorrelated retention in the two dimensioal "orthogonal" LCxLC separation systems.

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement
CC BY-NC-ND 4.0Creative CommonsAttributionNonCommercialNoDerivatives