Publication detail

CZE/LIF - A Powerful Tool for the Analysis of N-linked Glycans Released from Human Glycoproteins

Authors: Staněk Václav | Novotny Milos | Starkey Jason

Year: 2010

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	CZE/LIF - A Powerful Tool for the Analysis of N-linked Glycans Released from Human Glycoproteins	In present work, capillary zone electrophoresis (CZE) coupled with LIF detector as a powerful tool for identification of N-linked glycan structures derived from glycoproteins is demonstrated. Complex mixtures of derivatized N-glycans were resolved on a polyacrylamide coated capillary with reduced electroosmotic flow. To detect N-glycans using high sensitive LIF detection, a highly fluorescent dye 1-aminopyrene-3,6,8-trisulfonate (APTS) as a derivatization reagent was attached to the reducing end of oligosaccharide molecule through reductive amination. Glycan structure identification was elucidated using a combination of strategies, including comparison with standard glycan libraries and the enzymatic digestions of N-linked glycan pools using specific and non-specific exoglycosidases.

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