Publication detail

LC/MS Separation of Natural Antioxidants in Herbs and Honey Extracts using PFP Column

Authors: Dinisová Petra | Česla Petr | Česlová Lenka | Fischer Jan

Year: 2011

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	LC/MS Separation of Natural Antioxidants in Herbs and Honey Extracts using PFP Column	A LC/MS method has been developed for separation of polyphenolic compounds including phenolic acids and flavonoids in natural matrices. The composition of mobile phase, type of the column and gradient profile were optimized. Five different columns with C18, phenyl-hexyl and pentafluorophenyl (PFP) stationary phases were compared using mobile phases with methanol and acetonitrile mixtures with addition of 0,3% (v/v) formic acid. The best separation was achieved using column with PFP chemically bonded stationary phase. The segmented linear gradient profile, i.e. initial concentration of acetonitrile and gradient steepness in each segment, was optimized by numerical iteration procedure using regression parameters of experimental dependencies of retention factors of antioxidants under isocratic conditions on concentration of acetonitrile in mobile phase. The water extracts of flower of wild chamomile, lemon balm, sage and water extract of honey were analyzed under optimal conditions.	LC/MS; Natural Antioxidants; Herbs; Honey; PFP Column

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