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Capillary Electrophoresis in Multidimensional Separation Techniques For Analysis of Natural Compounds in Complex Matrices (A Review)
Authors: Česla Petr | Dinisová Petra | Fischer Jan
Year: 2011
Type of publication: článek ve sborníku
Name of source: Sensing in Electroanalysis Vol. 6
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 357-368
Titles:
Language Name Abstract Keywords
cze Kapilární elektroforéza ve vícerozměrných separačních technikách pro analýzy přírodních látek v komplexních matricích V kapitole jsou shrnuty experimentální aspekty a aplikace kapilární elektroforézy v 2D separačním systému se zaměřením zejména na kombinace s kapalinovou chromatografií. Kapilární elektroforéza; Micelární elektrokinetická kapilární chromatografie; Dvourozměrné separace; Ortogonalita; On-line zakoncentrování; Přírodní látky; Souhrný článek
eng Capillary Electrophoresis in Multidimensional Separation Techniques For Analysis of Natural Compounds in Complex Matrices (A Review) In past decades, capillary electromigration separation methods have been established as the routine techniques suitable for the analyzes of ionized and non-ionized compounds. The improvement in sensitivity of detection lead to discoveries of new substances presented at trace levels in various samples. For determination of such compounds, separation methods with high resolving power are needed, which leads to the rapid development of 2D methods. The article reviews some experimental aspects and applications of CE in the 2D separation systems, focused mainly to its combinations with LC. CE is being applied in 2D systems either in both or presumably in the 2nd dimension due to the speed of the analysis and injected volumes of samples. Advantages and drawbacks of the off-line and on-line experimental setups are discussed. Applications of 2D LC-CE are discussed with special emphasis on the possibilities of improvement of the detection sensitivity using in-capillary preconcentration techniques. Capillary electrophoresis; Micellar electrokinetic capillary chromatography; Two-dimensional separations; Orthogonality; On-line preconcentration; Natural compounds; Review

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