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Identification of Phase I and Phase II Metabolites of Benfluron and Dimefluron in Rat Urine Using High-performance Liquid Chromatography /Tandem Mass Spectrometry
Authors: Jirásko Robert | Holčapek Michal | Nobilis Milan
Year: 2011
Type of publication: článek v odborném periodiku
Name of source: Rapid Communication in Mass Spectrometry
Publisher name: John Wiley & Sons Ltd.
Place: Chichester
Page from-to: 2153-2162
Titles:
Language Name Abstract Keywords
cze Identifikace metabolitů 1. a 2. fáze benfluronu a dimefluronu v potkaních vzorcích s využitím HPLC/MS/MS S využitím HPLC/MS/MS byly charakterizovány 2 potenciální antikarcinogenní léčiva. Měření s vysokou správností m/z umožnilo potvrdit elementární složení nalezených metabolitů 1. a 2. fáze. Kombinací různých HPLC/MS/MS skenů bylo možné detegovat a následně identifikovat i méně zastoupené metabolity. Mezi typickými mechanismy tvorby metabolitů byla pozorována oxidace na aromatickém jédře, N-oxidace, O-demetylace, redukce karbonylové skupiny, glukuronidace a sulfatace. metabolity léčiv;HPLC/MS/MS;antineoplastika
eng Identification of Phase I and Phase II Metabolites of Benfluron and Dimefluron in Rat Urine Using High-performance Liquid Chromatography /Tandem Mass Spectrometry Biotransformation products of two potential antineoplastic agents are characterized using HPLC/UV/MS/MS. High mass accuracy measurement allows confirmation of an elemental composition and metabolic reactions according to exact mass defects. The combination of different HPLC/MS/MS scans, such as reconstructed ion current chromatograms, constant neutral loss chromatograms or exact mass filtration, helps the unambiguous detection of low abundance metabolites. The arene oxidation, N-oxidation, N-demethylation, O-demethylation, carbonyl reduction, glucuronidation and sulfation are typical mechanisms of the metabolite formation. The interpretation of their tandem mass spectra enables the distinction of demethylation position (N- vs. O-) as well as to differentiate N-oxidation from arene oxidation for both phase I and phase II metabolites. Two metabolic pathways are rather unusual for rat samples, i.e., glucosylation and double glucuronidation. antineoplastic agents;HPLC/MS/MS;drug metabolites

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