University of PardubiceFaculty of Chemical Technology

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UHPLC/MS/MS Metabolites Characterization of Flubendazole,

Authors: Jirásko Robert | Holčapek Michal | Jedličková Veronika | Nobilis Milan | Skálová Lenka | Lamka Jiří

Year: 2011

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	UHPLC/MS/MS Metabolites Characterization of Flubendazole,	Combination of our UHPLC separation with subsequent high mass accuracy measurement in full scan and tandem mass spectra modes using QqTOF analyzer enables the identification even for trace metabolites and give the reliable picture about present drug metabolites within several minutes. First, the data evaluation is performed and the elemental composition of present metabolites is determined based on the combination of following information: high mass accuracy, specific mass defect, characteristic neutral losses, comparison of fragmentation with parent drug or relative retention shifts. UHPLC/MS/MS detection of chromophore-containing metabolites in the complex mixture is improved by parallel UV detection. Advanced software tools are applied for the metabolite identification using the comparison of the blank chromatogram with the real incubation sample together with the software prediction and detection of possible metabolites.	flubendazole;UHPLC/MS/MS;drug metabolites