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Characterization of praziquantel and its metabolites by ultra-performance liguid chromatography coupled with electrospray tandem mass spectrometry
Authors: Jedličková Veronika | Jirásko Robert | Vokřál Ivan | Skálová Lenka | Holčapek Michal
Year: 2011
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Characterization of praziquantel and its metabolites by ultra-performance liguid chromatography coupled with electrospray tandem mass spectrometry In our work, PZQ was incubated with rat hepatocytes and microsomes. Subsequently, the solid-phase extraction of microsomal and hepatocyte incubation mixtures was performed. Moreover, rat urine samples were obtained from in vivo experiments after PZQ oral administration. The comparison of incubation samples with placebo experiments simplified the identification of metabolites. Studied samples were analyzed using optimized UHPLC coupled with electrospray ionization tandem mass spectrometry method that provided information about PZQ microsomal and hepatic biotransformation pathways. Structures of particular phase I and II metabolites were suggested based on the high mass accuracy measurements in full scan and tandem mass spectrometry modes using QqTOF analyzer. The additional measurements of MSn using ion trap analyzer were performed for the verification of fragmentation paths. praziquantel;drug metabolites;UHPLC/MS/MS

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