University of PardubiceFaculty of Chemical Technology

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Lipidomic analysis using off-line two-dimensional HILIC x RP-HPLC/MS

Authors: Cífková Eva | Lísa Miroslav | Holčapek Michal

Year: 2011

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Lipidomic analysis using off-line two-dimensional HILIC x RP-HPLC/MS	Lipids are important components in all biological tissues having many essential functions associated with the proper function of organisms. Lipidomics contributes towards the undestanding how lipids function in a biological system and for the elucidation of the mechanism of lipid-based diseases. For this purpose, the lipidomic analysis using off-line two-dimensional coupling of hydrophilic interaction liquid chromatography (HILIC) and reversed phase (RP) high-performance liquid chromatography mode with mass spectrometry detection was optimized. In the first dimension, total liquid extracts were fractioned using the HILIC separation into individual lipid classes. The fractions of individual lipid classes were collected and separated using RP-HPLC in the second dimension. Lipids were separated into individual lipid species according to the acyl lengths and number of double bonds. Off-line coupling of HILIC and RP-HPLC modes enables the separation of extremely high number of lipid species.	Lipidomics; Phospholipids; High interaction liquid chromatography; two-dimensional liquid chromatography