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Publication detail

Effect of Selected Fungicides on Fusarium Growth and toxins production
Authors: Hrubošová Dana | Vytřasová Jarmila | Moťková Petra
Year: 2011
Type of publication: článek v odborném periodiku
Name of source: Czech journal of food sciences
Publisher name: Česká akademie zemědělských věd
Place: Praha
Page from-to: 69-75
Titles:
Language Name Abstract Keywords
cze Vliv vybraných fungicidů na růst a produkci toxinů u plísní rodu Fusarium byl testován vliv vybraných fungicidů na růst plísní rodu Fusarium a produkci toxinů DON a T-2. Živná média s různými koncentracemi testovaných fungicidů byl inokulována plísněmi rodu Fusarium a inkubována za různých podmínek. Extrakce a stanovení fusariových toxinů bylo provedeno pomocí kitů Agra Quant®Deoxynivalenol Test kit and Agra Quant® T-2 toxin kit. genus Fusarium;mycotoxin;deoxynivalenol;T-2 toxin;fungicidy;PCR
eng Effect of Selected Fungicides on Fusarium Growth and toxins production We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested , inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant®Deoxynivalenol Test kit and Agra Quant® T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains was confirmed by PCR. The enzyme – trichodiene synthase – catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes. genus Fusarium;mycotoxin;deoxynivalenol;T-2 toxin; fungicide;PCR

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