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Temperature dependence of retention in reversed-phase and hydrophilic interaction liquid chromatography
Authors: Soukup Jan | Jandera Pavel
Year: 2011
Type of publication: článek v odborném periodiku
Name of source: Biologija
Publisher name: Lithuanian Academy of Sciences
Place: Vilnius
Page from-to: 85-91
Titles:
Language Name Abstract Keywords
cze Závislost retence na teplotě v chromatografii na reverzní fázi a v hydrofilní interakční kapalinové chromatografii Byl zkoumán vliv teploty na retenční mechanismus fenolických kyselin v chromatografii na reverzní fázi (RPLC) a v hydrofilní interakční kapalinové chromatografii (HILIC) na čytřech hydrosilovaných stacionárních fázích: hydrosilovaný silikagel, Diamond hydride, UDC cholesterolá a C18 bidentátová. Byly objeveny lineární závislosti retence na teplotě v HILIC i v RP módech na všech testovaných kolonách a byla použita van ´t Hoffova rovnice pro popis retenčního mechanismu. Úseky a směrnice lineárních zívislostí byly použity pro výpočty termodynamických konstant pro převod solutu z mobilní fáze do stacionární fáze (ΔH0 and ΔS0.
eng Temperature dependence of retention in reversed-phase and hydrophilic interaction liquid chromatography The retention mechanism in reversed-phase liquid chromatography (RPLC) and in hydrophilic interaction liquid chromatography (HILIC) have been investigated by examining the temperature dependence of retention of the chosen phenolic acids on two columns modified by hydrosilated silica – hydrosilated silica column, Diamond hydride column, cholesterol column and C18 bidentate column. The linear dependences of retention on temperature were observed in the HILIC as well as in the RPLC on all columns tested. Van’t Hoff equation was applied to describe the retention mechanism thoroughly, and its slopes and intercepts were used for calculations of thermodynamic constants for the transfer of a solute from the mobile phase to the stationary phase (ΔH0 and ΔS0). Phenolic acids; HILIC; van’t Hoff equation; Hydrosilated silica

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